Universität zu Köln

Xiao, Cally ORCID: 0000-0001-7172-3175, Grzonka, Marta, Meyer-Gerards, Charlotte, Mack, Miriam, Figge, Rebecca ORCID: 0000-0003-4141-5598 and Bazzi, Hisham ORCID: 0000-0001-8388-4005 (2021). Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development. EMBO Rep., 22 (2). HOBOKEN: WILEY. ISSN 1469-3178

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Abstract

Centrosomes, composed of two centrioles and pericentriolar material, organize mitotic spindles during cell division and template cilia during interphase. The first few divisions during mouse development occur without centrioles, which form around embryonic day (E) 3. However, disruption of centriole biogenesis in Sas-4 null mice leads to embryonic arrest around E9. Centriole loss in Sas-4(-/-) embryos causes prolonged mitosis and p53-dependent cell death. Studies in vitro discovered a similar USP28-, 53BP1-, and p53-dependent mitotic surveillance pathway that leads to cell cycle arrest. In this study, we show that an analogous pathway is conserved in vivo where 53BP1 and USP28 are upstream of p53 in Sas-4(-/-) embryos. The data indicate that the pathway is established around E7 of development, four days after the centrioles appear. Our data suggest that the newly formed centrioles gradually mature to participate in mitosis and cilia formation around the beginning of gastrulation, coinciding with the activation of mitotic surveillance pathway upon centriole loss.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Xiao, Cally UNSPECIFIED orcid.org/0000-0001-7172-3175 UNSPECIFIED Grzonka, Marta UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer-Gerards, Charlotte UNSPECIFIED UNSPECIFIED UNSPECIFIED Mack, Miriam UNSPECIFIED UNSPECIFIED UNSPECIFIED Figge, Rebecca UNSPECIFIED orcid.org/0000-0003-4141-5598 UNSPECIFIED Bazzi, Hisham UNSPECIFIED orcid.org/0000-0001-8388-4005 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-576394
DOI:	10.15252/embr.202051127
Journal or Publication Title:	EMBO Rep.
Volume:	22
Number:	2
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-3178
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PREIMPLANTATION DEVELOPMENT; CENTROSOME LOSS; C-ELEGANS; PROTEIN; DUPLICATION; 53BP1; CELLS; SAS-4; MODE Multiple languages Biochemistry & Molecular Biology; Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57639