Universität zu Köln

Baranek, Miroslav, Kovacova, Viera ORCID: 0000-0003-4581-4254, Gazdik, Filip, Spetik, Milan, Eichmeier, Ales, Pulawska, Joanna ORCID: 0000-0001-5327-1056 and Barankova, Katerina ORCID: 0000-0002-9965-4666 (2021). Epigenetic Modulating Chemicals Significantly Affect the Virulence and Genetic Characteristics of the Bacterial Plant Pathogen Xanthomonas campestris pv. campestris. Genes, 12 (6). BASEL: MDPI. ISSN 2073-4425

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Abstract

Epigenetics is the study of heritable alterations in phenotypes that are not caused by changes in DNA sequence. In the present study, we characterized the genetic and phenotypic alterations of the bacterial plant pathogen Xanthomonas campestris pv. campestris (Xcc) under different treatments with several epigenetic modulating chemicals. The use of DNA demethylating chemicals unambiguously caused a durable decrease in Xcc bacterial virulence, even after its reisolation from infected plants. The first-time use of chemicals to modify the activity of sirtuins also showed some noticeable results in terms of increasing bacterial virulence, but this effect was not typically stable. Changes in treated strains were also confirmed by using methylation sensitive amplification (MSAP), but with respect to registered SNPs induction, it was necessary to consider their contribution to the observed polymorphism. The molecular basis of the altered virulence was deciphered by using dualRNA-seq analysis of treated Xcc strains infecting Brassica rapa plants. The results of the present study should promote more intensive research in the generally understudied field of bacterial epigenetics, where artificially induced modification by epigenetic modulating chemicals can significantly increase the diversity of bacterial properties and potentially contribute to the further development of the fields, such as bacterial ecology and adaptation.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Baranek, Miroslav UNSPECIFIED UNSPECIFIED UNSPECIFIED Kovacova, Viera UNSPECIFIED orcid.org/0000-0003-4581-4254 UNSPECIFIED Gazdik, Filip UNSPECIFIED UNSPECIFIED UNSPECIFIED Spetik, Milan UNSPECIFIED UNSPECIFIED UNSPECIFIED Eichmeier, Ales UNSPECIFIED UNSPECIFIED UNSPECIFIED Pulawska, Joanna UNSPECIFIED orcid.org/0000-0001-5327-1056 UNSPECIFIED Barankova, Katerina UNSPECIFIED orcid.org/0000-0002-9965-4666 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-577872
DOI:	10.3390/genes12060804
Journal or Publication Title:	Genes
Volume:	12
Number:	6
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4425
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language RESTRICTION-MODIFICATION SYSTEMS; DNA METHYLATION; 5-AZACYTIDINE; ROLES; IDENTIFICATION; ACETYLATION; INHIBITION; EXPRESSION; SIRTUINS; NITROGEN Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/57787