Universität zu Köln

Wenske, Sebastian, Lackmann, Jan-Wilm ORCID: 0000-0001-8182-8034, Busch, Larissa Milena, Bekeschus, Sander, von Woedtke, Thomas and Wende, Kristian ORCID: 0000-0001-5217-0683 (2021). Reactive species driven oxidative modifications of peptides-Tracing physical plasma liquid chemistry. J. Appl. Phys., 129 (19). MELVILLE: AIP Publishing. ISSN 1089-7550

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Abstract

The effluence of physical plasma consists of a significant share of reactive species, which may interact with biomolecules and yield chemical modifications comparable to those of physiological processes, e.g., post-translational protein modifications (oxPTMs). Consequentially, the aim of this work is to understand the role of physical plasma-derived reactive species in the introduction of oxPTM-like modifications in proteins. An artificial peptide library consisting of ten peptides was screened against the impact of two plasma sources, the argon-driven MHz-jet kINPen and the helium-driven RF-jet COST-Jet. Changes in the peptide molecular structure were analyzed by liquid chromatography-mass spectrometry. The amino acids cysteine, methionine, tyrosine, and tryptophan were identified as major targets. The introduction of one, two, or three oxygen atoms was the most common modification observed. Distinct modification patterns were observed for nitration (+N+2O-H), which occurred in kINPen only (peroxynitrite), and chlorination (+Cl-H) that was exclusive for the COST-Jet in the presence of chloride ions (atomic oxygen/hypochlorite). Predominantly for the kINPen, singlet oxygen-related modifications, e.g., cleavage of tryptophan, were observed. Oxidation, carbonylation, and double oxidations were attributed to the impact of hydroxyl radicals and atomic oxygen. Leading to a significant change in the peptide side chain, most of these oxPTM-like modifications affect the secondary structure of amino acid chains, and amino acid polarity/functionality, ultimately modifying the performance and stability of cellular proteins. (c) 2021 Author(s).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wenske, Sebastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Lackmann, Jan-Wilm UNSPECIFIED orcid.org/0000-0001-8182-8034 UNSPECIFIED Busch, Larissa Milena UNSPECIFIED UNSPECIFIED UNSPECIFIED Bekeschus, Sander UNSPECIFIED UNSPECIFIED UNSPECIFIED von Woedtke, Thomas UNSPECIFIED UNSPECIFIED UNSPECIFIED Wende, Kristian UNSPECIFIED orcid.org/0000-0001-5217-0683 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-580117
DOI:	10.1063/5.0046685
Journal or Publication Title:	J. Appl. Phys.
Volume:	129
Number:	19
Date:	2021
Publisher:	AIP Publishing
Place of Publication:	MELVILLE
ISSN:	1089-7550
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POSTTRANSLATIONAL MODIFICATIONS; COLD-PLASMA; OXYGEN; IDENTIFICATION; PEROXYNITRITE; HYDROXYLATION; MECHANISMS; GENERATION; TRYPTOPHAN; NITRATION Multiple languages Physics, Applied Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58011