Fukusato, Shin, Nagao, Masashi ORCID: 0000-0002-8909-4650, Fujihara, Kei, Yoneda, Taiju, Arai, Kiyotaka, Koch, Manuel ORCID: 0000-0002-2962-7814, Kaneko, Kazuo, Ishijima, Muneaki and Izu, Yayoi (2021). Collagen XII Deficiency Increases the Risk of Anterior Cruciate Ligament Injury in Mice. J. Clin. Med., 10 (18). BASEL: MDPI. ISSN 2077-0383

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Abstract

Anterior cruciate ligament (ACL) rupture is a common knee injury for athletes. Although surgical reconstruction is recommended for the treatment of ACL ruptures, 100% functional recovery is unlikely. Therefore, the discovery of risk factors for ACL ruptures may prevent injury. Several studies have reported an association between polymorphisms of the collagen XII gene COL12A1 and ACL rupture. Collagen XII is highly expressed in tendons and ligaments and regulates tissue structure and mechanical property. Therefore, we hypothesized that collagen XII deficiency may cause ACL injury. To elucidate the influence of collagen XII deficiency on ACL, we analyzed a mouse model deficient for Col12a1. Four- to 19-week-old male Col12a1(-/-) and wild-type control mice were used for gait analysis; histological and immunofluorescent analysis of collagen XII, and real-time RT-PCR evaluation of Col12a1 mRNA expression. The Col12a1(-/-) mice showed an abnormal gait with an approximately 2.7-fold increase in step angle, suggesting altered step alignment. Col12a1(-/-) mice displayed 20-60% ACL discontinuities, but 0% discontinuity in the posterior cruciate ligament. No discontinuities in knee ligaments were found in wild-type mice. Collagen XII mRNA expression in the ACL tended to decrease with aging. Our study demonstrates for the first time that collagen XII deficiency increases the risk of ACL injury.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Fukusato, ShinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nagao, MasashiUNSPECIFIEDorcid.org/0000-0002-8909-4650UNSPECIFIED
Fujihara, KeiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Yoneda, TaijuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Arai, KiyotakaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Koch, ManuelUNSPECIFIEDorcid.org/0000-0002-2962-7814UNSPECIFIED
Kaneko, KazuoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ishijima, MuneakiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Izu, YayoiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-580425
DOI: 10.3390/jcm10184051
Journal or Publication Title: J. Clin. Med.
Volume: 10
Number: 18
Date: 2021
Publisher: MDPI
Place of Publication: BASEL
ISSN: 2077-0383
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
TENDON; GENE; BONE; RECONSTRUCTION; IDENTIFICATION; EXPRESSION; MUTATIONS; PARTNERS; COL12A1Multiple languages
Medicine, General & InternalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/58042

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