Universität zu Köln

Watermann, Christoph, Meyer, Malin T., Valerius, Klaus P., Kleefeldt, Florian, Wagner, Steffen ORCID: 0000-0003-0873-1601, Wittekindt, Claus, Klussmann, Jens P., Erguen, Suleyman, Baumgart-Vogt, Eveline and Karnati, Srikanth (2021). Peroxisomes in the mouse parotid glands: An in-depth morphological and molecular analysis. Ann. Anat.-Anat. Anz., 238. MUNICH: ELSEVIER GMBH. ISSN 1618-0402

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Abstract

Background: The parotid gland is a major salivary gland that has important roles in the digestive and immune system. Peroxisomes are ubiquitous, single-membrane-bound organelles that are present in all eukaryotic cells. Peroxisomes help mediate lipid and reactive oxygen species metabolism, as well as polyunsaturated fatty acid, cholesterol and plasmalogen synthesis. Much of the knowledge on peroxisomes has derived from metabolic organs, however no detailed knowledge is available on peroxisomes in the parotid glands. We thus aimed to comprehensively delineate the localization and characterization of peroxisomal proteins in the murine parotid gland. Methods: We characterized peroxisomes in the acinar and striated duct cells of the murine parotid gland by fluorescence and electron microscopy, as well as protein and mRNA expression analyses for important peroxisomal genes and proteins. Results: We found that peroxisomes are present in all cell types of the mouse parotid gland, however, exhibit notable cell-specific differences in their abundance and enzyme content. We also observed that mouse parotid glands contain high levels of peroxisomal beta-oxidation enzymes (including Acox1, Mfp2 and Acaa1), catalase and other peroxisomal anti-oxidative enzymes. Conclusions: This data suggests that peroxisomes are highly abundant in the murine parotid gland and might help to protect against oxidative stress. This comprehensive description of peroxisomes in the parotid gland lays the groundwork for further research concerning their role in the pathogenesis of parotid gland diseases and tumors. (C) 2021 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Watermann, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Meyer, Malin T. UNSPECIFIED UNSPECIFIED UNSPECIFIED Valerius, Klaus P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Kleefeldt, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagner, Steffen UNSPECIFIED orcid.org/0000-0003-0873-1601 UNSPECIFIED Wittekindt, Claus UNSPECIFIED UNSPECIFIED UNSPECIFIED Klussmann, Jens P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Erguen, Suleyman UNSPECIFIED UNSPECIFIED UNSPECIFIED Baumgart-Vogt, Eveline UNSPECIFIED UNSPECIFIED UNSPECIFIED Karnati, Srikanth UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-582122
DOI:	10.1016/j.aanat.2021.151778
Journal or Publication Title:	Ann. Anat.-Anat. Anz.
Volume:	238
Date:	2021
Publisher:	ELSEVIER GMBH
Place of Publication:	MUNICH
ISSN:	1618-0402
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DIFFERENTIAL EXPRESSION; POSTNATAL-DEVELOPMENT; OXIDATIVE STRESS; SALIVARY-GLANDS; BETA-OXIDATION; CELL-TYPES; PROTEIN; LOCALIZATION; PEROXIDASE; ULTRASTRUCTURE Multiple languages Anatomy & Morphology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58212