Doria, Halina Binde, Waldvogel, Ann-Marie and Pfenninger, Markus 
ORCID: 0000-0002-1547-7245
(2021).
Measuring mutagenicity in ecotoxicology: A case study of Cd exposure in Chironomus riparius.
Environ. Pollut., 272.
OXFORD:
ELSEVIER SCI LTD.
ISSN 1873-6424
Abstract
Existing mutagenicity tests for metazoans lack the direct observation of enhanced germline mutation rates after exposure to anthropogenic substances, therefore being inefficient. Cadmium (Cd) is a metal described as a mutagen in mammalian cells and listed as a group 1 carcinogenic and mutagenic substance. But Cd mutagenesis mechanism is not yet clear. Therefore, in the present study, we propose a method coupling short-term mutation accumulation (MA) lines with subsequent whole genome sequencing (WGS) and a dedicated data analysis pipeline to investigate if chronic Cd exposure on Chironomus riparius can alter the rate at which de novo point mutations appear. Results show that Cd exposure did not affect the basal germline mutation rate nor the mutational spectrum in C. riparius, thereby arguing that exposed organisms might experience a range of other toxic effects before any mutagenic effect may occur. We show that it is possible to establish a practical and easily implemented pipeline to rapidly detect germ cell mutagens in a metazoan test organism. Furthermore, our data implicate that it is questionable to transfer mutagenicity assessments based on in vitro methods to complex metazoans. (C) 2020 Elsevier Ltd. All rights reserved.
| Item Type: | Journal Article | ||||||||||||||||
| Creators: |
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| URN: | urn:nbn:de:hbz:38-582166 | ||||||||||||||||
| DOI: | 10.1016/j.envpol.2020.116004 | ||||||||||||||||
| Journal or Publication Title: | Environ. Pollut. | ||||||||||||||||
| Volume: | 272 | ||||||||||||||||
| Date: | 2021 | ||||||||||||||||
| Publisher: | ELSEVIER SCI LTD | ||||||||||||||||
| Place of Publication: | OXFORD | ||||||||||||||||
| ISSN: | 1873-6424 | ||||||||||||||||
| Language: | English | ||||||||||||||||
| Faculty: | Unspecified | ||||||||||||||||
| Divisions: | Unspecified | ||||||||||||||||
| Subjects: | no entry | ||||||||||||||||
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| URI: | http://kups.ub.uni-koeln.de/id/eprint/58216 |
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