Universität zu Köln

Cosentino, Katia, Hermann, Edward, von Kugelgen, Nicolai, Unsay, Joseph D., Ros, Uris and Garcia-Saez, Ana J. (2021). Force Mapping Study of Actinoporin Effect in Membranes Presenting Phase Domains. Toxins, 13 (9). BASEL: MDPI. ISSN 2072-6651

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Abstract

Equinatoxin II (EqtII) and Fragaceatoxin C (FraC) are pore-forming toxins (PFTs) from the actinoporin family that have enhanced membrane affinity in the presence of sphingomyelin (SM) and phase coexistence in the membrane. However, little is known about the effect of these proteins on the nanoscopic properties of membrane domains. Here, we used combined confocal microscopy and force mapping by atomic force microscopy to study the effect of EqtII and FraC on the organization of phase-separated phosphatidylcholine/SM/cholesterol membranes. To this aim, we developed a fast, high-throughput processing tool to correlate structural and nano-mechanical information from force mapping. We found that both proteins changed the lipid domain shape. Strikingly, they induced a reduction in the domain area and circularity, suggesting a decrease in the line tension due to a lipid phase height mismatch, which correlated with proteins binding to the domain interfaces. Moreover, force mapping suggested that the proteins affected the mechanical properties at the edge, but not in the bulk, of the domains. This effect could not be revealed by ensemble force spectroscopy measurements supporting the suitability of force mapping to study local membrane topographical and mechanical alterations by membranotropic proteins.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Cosentino, Katia UNSPECIFIED UNSPECIFIED UNSPECIFIED Hermann, Edward UNSPECIFIED UNSPECIFIED UNSPECIFIED von Kugelgen, Nicolai UNSPECIFIED UNSPECIFIED UNSPECIFIED Unsay, Joseph D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Ros, Uris UNSPECIFIED UNSPECIFIED UNSPECIFIED Garcia-Saez, Ana J. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-582225
DOI:	10.3390/toxins13090669
Journal or Publication Title:	Toxins
Volume:	13
Number:	9
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2072-6651
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PORE-FORMING TOXIN; SUPPORTED LIPID-BILAYERS; EQUINATOXIN-II; LINE TENSION; SPHINGOMYELIN; OLIGOMERIZATION; CHOLESTEROL; MICROSCOPY; CURVATURE; MECHANISM Multiple languages Food Science & Technology; Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58222