Klupp, Fee, Kahlert, Christoph, Franz, Clemens, Halama, Niels, Schleussner, Nikolai, Wirsik, Naita M., Warth, Arne, Schmidt, Thomas ORCID: 0000-0002-7166-3675 and Ulrich, Alexis B. (2021). Granulin: An Invasive and Survival-Determining Marker in Colorectal Cancer Patients. Int. J. Mol. Sci., 22 (12). BASEL: MDPI. ISSN 1422-0067

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Abstract

Background: Granulin is a secreted, glycosylated peptide-originated by cleavage from a precursor protein-which is involved in cell growth, tumor invasion and angiogenesis. However, the specific prognostic impact of granulin in human colorectal cancer has only been studied to a limited extent. Thus, we wanted to assess the expression of granulin in colorectal cancer patients to evaluate its potential as a prognostic biomarker. Methods: Expressional differences of granulin in colorectal carcinoma tissue (n = 94) and corresponding healthy colon mucosa were assessed using qRT-PCR. Immunohistochemistry was performed in colorectal cancer specimens (n = 97), corresponding healthy mucosa (n = 47) and colorectal adenomas (n = 19). Subsequently, the results were correlated with histopathological and clinical patients' data. HCT-116 cells were transfected with siRNA for invasion and migration assays. Results: Immunohistochemistry and qRT-PCR revealed tumoral over expression of granulin in colorectal cancer specimens compared to corresponding healthy colon mucosa and adenomas. Tumoral overexpression of granulin was associated with a significantly impaired overall survival. Moreover, downregulation of granulin by siRNA significantly diminished the invasive capacities of HCT-116 cells in vitro. Conclusion: Expression of granulin differs in colorectal cancer tissue, adenomas and healthy colon mucosa. Furthermore, granulin features invasive and migrative capabilities and overexpression of granulin correlates with a dismal prognosis. This reveals its potential as a prognostic biomarker and granulin could be a worthwhile molecular target for individualized anticancer therapy.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Klupp, FeeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kahlert, ChristophUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Franz, ClemensUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Halama, NielsUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schleussner, NikolaiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wirsik, Naita M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Warth, ArneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schmidt, ThomasUNSPECIFIEDorcid.org/0000-0002-7166-3675UNSPECIFIED
Ulrich, Alexis B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-582340
DOI: 10.3390/ijms22126436
Journal or Publication Title: Int. J. Mol. Sci.
Volume: 22
Number: 12
Date: 2021
Publisher: MDPI
Place of Publication: BASEL
ISSN: 1422-0067
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EPITHELIN PRECURSOR; GROWTH-FACTOR; HEPATOCELLULAR-CARCINOMA; PROGRANULIN; PROMOTES; CELLS; EXPRESSION; PROEPITHELIN; INFLAMMATION; CROSSROADSMultiple languages
Biochemistry & Molecular Biology; Chemistry, MultidisciplinaryMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/58234

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