Universität zu Köln

Trela, Ewelina ORCID: 0000-0002-8374-6136, Lan, Qiang ORCID: 0000-0002-7765-6767, Myllymaki, Satu-Marja ORCID: 0000-0003-0521-5062, Villeneuve, Clementine, Lindstrom, Riitta ORCID: 0000-0001-5177-0564, Kumar, Vinod ORCID: 0000-0002-7167-0402, Wickstrom, Sara A. and Mikkola, Marja L. (2021). Cell influx and contractile actomyosin force drive mammary bud growth and invagination. J. Cell Biol., 220 (8). NEW YORK: ROCKEFELLER UNIV PRESS. ISSN 1540-8140

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Abstract

The mammary gland develops from the surface ectoderm during embryogenesis and proceeds through morphological phases defined as placode, hillock, bud, and bulb stages followed by branching morphogenesis. During this early morphogenesis, the mammary bud undergoes an invagination process where the thickened bud initially protrudes above the surface epithelium and then transforms to a bulb and sinks into the underlying mesenchyme. The signaling pathways regulating the early morphogenetic steps have been identified to some extent, but the underlying cellular mechanisms remain ill defined. Here, we use 3D and 4D confocal microscopy to show that the early growth of the mammary rudiment is accomplished by migration-driven cell influx, with minor contributions of cell hypertrophy and proliferation. We delineate a hitherto undescribed invagination mechanism driven by thin, elongated keratinocytes-ring cells-that form a contractile rim around the mammary bud and likely exert force via the actomyosin network. Furthermore, we show that conditional deletion of nonmuscle myosin IIA (NMIIA) impairs invagination, resulting in abnormal mammary bud shape.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Trela, Ewelina UNSPECIFIED orcid.org/0000-0002-8374-6136 UNSPECIFIED Lan, Qiang UNSPECIFIED orcid.org/0000-0002-7765-6767 UNSPECIFIED Myllymaki, Satu-Marja UNSPECIFIED orcid.org/0000-0003-0521-5062 UNSPECIFIED Villeneuve, Clementine UNSPECIFIED UNSPECIFIED UNSPECIFIED Lindstrom, Riitta UNSPECIFIED orcid.org/0000-0001-5177-0564 UNSPECIFIED Kumar, Vinod UNSPECIFIED orcid.org/0000-0002-7167-0402 UNSPECIFIED Wickstrom, Sara A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mikkola, Marja L. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-583013
DOI:	10.1083/jcb.202008062
Journal or Publication Title:	J. Cell Biol.
Volume:	220
Number:	8
Date:	2021
Publisher:	ROCKEFELLER UNIV PRESS
Place of Publication:	NEW YORK
ISSN:	1540-8140
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SONIC-HEDGEHOG; MOUSE; PROLIFERATION; MORPHOGENESIS; LINE; DIFFERENTIATION; MORPHOLOGY; MIGRATION; POLARITY; ROLES Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58301