Universität zu Köln

Fabri, Mario, Villa, Matteo ORCID: 0000-0003-2684-0596, Stanczak, Michal A., Edwards-Hicks, Joy ORCID: 0000-0002-9313-5718, Corrado, Mauro ORCID: 0000-0002-0617-3274 and Pearce, Erika L. (2021). Research Techniques Made Simple: Profiling Cellular Energy Metabolism. J. Invest. Dermatol., 141 (12). S. 2767 - 2777. NEW YORK: ELSEVIER SCIENCE INC. ISSN 1523-1747

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Abstract

The analysis of cellular metabolism is attracting increasing interest. Glycolysis and oxidative phosphorylation are intertwined with one another and dozens of other pathways to ultimately produce energy and maintain cellular fitness. However, cellular metabolism is much more than this. Metabolism underlies the proliferation, differentiation, and function of cells as well as the coordination of intercellular communication. Investigating metabolism allows the interpretation of cellular behavior in health and disease. In this article, we aim to demystify the complexity of cellular metabolism and explain the common approaches to study it. Whereas the analysis of cellular metabolism by western blot or flow cytometry might be accessible to most investigators, the functional and comprehensive analyses obtained with a Seahorse Analyzer or mass spectrometer come with monetary and logistical hurdles. We believe that the application of these techniques, together with collaborative efforts between scientists and clinicians, will uncover disease mechanisms and open novel therapeutic avenues for unmet clinical needs in the field of dermatology.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Fabri, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Villa, Matteo UNSPECIFIED orcid.org/0000-0003-2684-0596 UNSPECIFIED Stanczak, Michal A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Edwards-Hicks, Joy UNSPECIFIED orcid.org/0000-0002-9313-5718 UNSPECIFIED Corrado, Mauro UNSPECIFIED orcid.org/0000-0002-0617-3274 UNSPECIFIED Pearce, Erika L. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-583106
DOI:	10.1016/j.jid.2021.09.004
Journal or Publication Title:	J. Invest. Dermatol.
Volume:	141
Number:	12
Page Range:	S. 2767 - 2777
Date:	2021
Publisher:	ELSEVIER SCIENCE INC
Place of Publication:	NEW YORK
ISSN:	1523-1747
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SIMPLE EXPERIMENTAL METHODOLOGY; METABOLOMICS Multiple languages Dermatology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58310