Universität zu Köln

Passarge, Andrea, Demir, Fatih ORCID: 0000-0002-5744-0205, Green, Kimberly, Depotter, Jasper R. L., Scott, Barry ORCID: 0000-0002-2224-2946, Huesgen, Pitter F., Doehlemann, Gunther ORCID: 0000-0002-7353-8456 and Misas Villamil, Johana C. (2021). Host apoplastic cysteine protease activity is suppressed during the mutualistic association of Lolium perenne and Epichloe festucae. J. Exp. Bot., 72 (9). S. 3410 - 3427. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431

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Abstract

Plants secrete various defence-related proteins into the apoplast, including proteases. Papain-like cysteine proteases (PLCPs) are central components of the plant immune system. To overcome plant immunity and successfully colonize their hosts, several plant pathogens secrete effector proteins inhibiting plant PLCPs. We hypothesized that not only pathogens, but also mutualistic microorganisms interfere with PLCP-meditated plant defences to maintain endophytic colonization with their hosts. Epichloe festucae forms mutualistic associations with cool season grasses and produces a range of secondary metabolites that protect the host against herbivores. In this study, we performed a genome-wide identification of Lolium perenne PLCPs, analysed their evolutionary relationship, and classified them into nine PLCP subfamilies. Using activity-based protein profiling, we identified four active PLCPs in the apoplast of L. perenne leaves that are inhibited during endophyte interactions. We characterized the L. perenne cystatin LpCys1 for its inhibitory capacity against ryegrass PLCPs. LpCys1 abundance is not altered during the mutualistic interaction and it mainly inhibits LpCP2. However, since the activity of other L. perenne PLCPs is not sensitive to LpCys1, we propose that additional inhibitors, likely of fungal origin, are involved in the suppression of apoplastic PLCPs during E. festucae infection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Passarge, Andrea UNSPECIFIED UNSPECIFIED UNSPECIFIED Demir, Fatih UNSPECIFIED orcid.org/0000-0002-5744-0205 UNSPECIFIED Green, Kimberly UNSPECIFIED UNSPECIFIED UNSPECIFIED Depotter, Jasper R. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Scott, Barry UNSPECIFIED orcid.org/0000-0002-2224-2946 UNSPECIFIED Huesgen, Pitter F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Doehlemann, Gunther UNSPECIFIED orcid.org/0000-0002-7353-8456 UNSPECIFIED Misas Villamil, Johana C. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-583180
DOI:	10.1093/jxb/erab088
Journal or Publication Title:	J. Exp. Bot.
Volume:	72
Number:	9
Page Range:	S. 3410 - 3427
Date:	2021
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2431
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CYSTATIN GENE FAMILY; DISEASE RESISTANCE; CATHEPSIN-B; HYPERSENSITIVE RESPONSE; PHYTOPHTHORA-INFESTANS; LACCARIA-BICOLOR; IMMUNE PROTEASE; TALL FESCUE; EFFECTOR; RYEGRASS Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58318