Universität zu Köln

Kraemer, Jonas, Peigneur, Steve, Tytgat, Jan, Jenner, Ronald A., van Toor, Ronald ORCID: 0000-0003-4190-5589 and Predel, Reinhard (2021). A Pseudoscorpion's Promising Pinch: The venom of Chelifer cancroides contains a rich source of novel compounds. Toxicon, 201. S. 92 - 105. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1879-3150

Full text not available from this repository.

Abstract

With pedipalps modified for venom injection, some pseudoscorpions possess a unique venom delivery system, which evolved independently from those of other arachnids like scorpions and spiders. Up to now, only a few studies have been focused on pseudoscorpion venom, which either identified a small fraction of venom compounds, or were based on solely transcriptomic approaches. Only one study addressed the bioactivity of pseudoscorpion venom. Here, we expand existing knowledge about pseudoscorpion venom by providing a comprehensive proteomic and transcriptomic analysis of the venom of Chelifer cancroides. We identified the first putative genuine toxins in the venom of C. cancroides and we showed that a large fraction of the venom comprises novel compounds. In addition, we tested the activity of the venom at specific ion channels for the first time. These tests demonstrate that the venom of C. cancroides causes inhibition of a voltage-gated insect potassium channel (Shaker IR) and modulates the inactivation process of voltage-gated sodium channels from Varroa destructor. For one of the smallest venomous animals ever studied, today's toolkits enabled a comprehensive venom analysis. This is demonstrated by allocating our identified venom compounds to more than half of the prominent ion signals in MALDI-TOF mass spectra of venom samples. The present study is a starting point for understanding the complex composition and activity of pseudoscorpion venom and provides a potential rich source of bioactive compounds useable for basic research and industrial application.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kraemer, Jonas UNSPECIFIED UNSPECIFIED UNSPECIFIED Peigneur, Steve UNSPECIFIED UNSPECIFIED UNSPECIFIED Tytgat, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Jenner, Ronald A. UNSPECIFIED UNSPECIFIED UNSPECIFIED van Toor, Ronald UNSPECIFIED orcid.org/0000-0003-4190-5589 UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-585502
DOI:	10.1016/j.toxicon.2021.08.012
Journal or Publication Title:	Toxicon
Volume:	201
Page Range:	S. 92 - 105
Date:	2021
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1879-3150
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language METALLOPROTEASES; TRANSCRIPTOMICS; MECHANISM; COCKTAIL; DEFENSIN; SEQUENCE; TOXINS Multiple languages Pharmacology & Pharmacy; Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58550