Universität zu Köln

Goeschl, Lorenz, Gmeiner, Guenter, Gaertner, Peter ORCID: 0000-0003-1569-5020, Stadler, Georg, Enev, Valentin, Thevis, Mario, Schaenzer, Wilhelm, Guddat, Sven and Forsdahl, Guro (2021). Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis. Drug Test. Anal., 13 (9). S. 1668 - 1678. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

The exogenous anabolic-androgenic steroid (AAS) stanozolol stays one of the most detected substances in professional sports. Its detection is a fundamental part of doping analysis, and the analysis of this steroid has been intensively investigated for a long time. This contribution to the detection of stanozolol doping describes for the first time the unambiguous proof for the existence of 17-epistanozolol-1 ' N-glucuronide and 17-epistanozolol-2 ' N-glucuronide in stanozolol-positive human urine samples due to the access to high-quality reference standards. Examination of excretion study samples shows large detection windows for the phase-II metabolites stanozolol-1 ' N-glucuronide and 17-epistanozolol-1 ' N-glucuronide up to 12 days and respectively up to almost 28 days. In addition, we present appropriate validation parameters for the analysis of these metabolites using a fully automatic method online solid-phase extraction (SPE) method already published before. Limits of identification (LOIs) as low as 100 pg/ml and other validation parameters like accuracy, precision, sensitivity, robustness, and linearity are given.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Goeschl, Lorenz UNSPECIFIED UNSPECIFIED UNSPECIFIED Gmeiner, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED Gaertner, Peter UNSPECIFIED orcid.org/0000-0003-1569-5020 UNSPECIFIED Stadler, Georg UNSPECIFIED UNSPECIFIED UNSPECIFIED Enev, Valentin UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Guddat, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Forsdahl, Guro UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-585979
DOI:	10.1002/dta.3109
Journal or Publication Title:	Drug Test. Anal.
Volume:	13
Number:	9
Page Range:	S. 1668 - 1678
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1942-7611
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EXPANDING ANALYTICAL POSSIBILITIES; MASS-SPECTROMETRIC DETECTION; ANABOLIC-STEROIDS; ELECTROSPRAY-IONIZATION; GC-MS; STRATEGY; MARKERS; MISUSE; MS/MS Multiple languages Biochemical Research Methods; Chemistry, Analytical; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58597