Goeschl, Lorenz, Gmeiner, Guenter, Gaertner, Peter ORCID: 0000-0003-1569-5020, Stadler, Georg, Enev, Valentin, Thevis, Mario, Schaenzer, Wilhelm, Guddat, Sven and Forsdahl, Guro (2021). Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis. Drug Test. Anal., 13 (9). S. 1668 - 1678. HOBOKEN: WILEY. ISSN 1942-7611

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Abstract

The exogenous anabolic-androgenic steroid (AAS) stanozolol stays one of the most detected substances in professional sports. Its detection is a fundamental part of doping analysis, and the analysis of this steroid has been intensively investigated for a long time. This contribution to the detection of stanozolol doping describes for the first time the unambiguous proof for the existence of 17-epistanozolol-1 ' N-glucuronide and 17-epistanozolol-2 ' N-glucuronide in stanozolol-positive human urine samples due to the access to high-quality reference standards. Examination of excretion study samples shows large detection windows for the phase-II metabolites stanozolol-1 ' N-glucuronide and 17-epistanozolol-1 ' N-glucuronide up to 12 days and respectively up to almost 28 days. In addition, we present appropriate validation parameters for the analysis of these metabolites using a fully automatic method online solid-phase extraction (SPE) method already published before. Limits of identification (LOIs) as low as 100 pg/ml and other validation parameters like accuracy, precision, sensitivity, robustness, and linearity are given.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Goeschl, LorenzUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gmeiner, GuenterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gaertner, PeterUNSPECIFIEDorcid.org/0000-0003-1569-5020UNSPECIFIED
Stadler, GeorgUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Enev, ValentinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Thevis, MarioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schaenzer, WilhelmUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Guddat, SvenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Forsdahl, GuroUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-585979
DOI: 10.1002/dta.3109
Journal or Publication Title: Drug Test. Anal.
Volume: 13
Number: 9
Page Range: S. 1668 - 1678
Date: 2021
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1942-7611
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
EXPANDING ANALYTICAL POSSIBILITIES; MASS-SPECTROMETRIC DETECTION; ANABOLIC-STEROIDS; ELECTROSPRAY-IONIZATION; GC-MS; STRATEGY; MARKERS; MISUSE; MS/MSMultiple languages
Biochemical Research Methods; Chemistry, Analytical; Pharmacology & PharmacyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/58597

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