Universität zu Köln

Schroeder, Daniel C., Popp, Erik, Rohleder, Cathrin ORCID: 0000-0002-3559-1846, Vus, Stefanie, Bethencourt, David de la Puente, Finke, Simon R., Zlatopolskiy, Boris D., Zischler, Johannes, Drzezga, Alexander ORCID: 0000-0001-6018-716X, Herff, Holger, Annecke, Thorsten, Hucho, Tim ORCID: 0000-0002-4147-9308, Neumaier, Bernd, Boettiger, Bernd W. and Endepols, Heike (2021). Positron Emission Tomography Imaging of Long-Term Expression of the 18 kDa Translocator Protein After Sudden Cardiac Arrest in Rats. Shock, 55 (5). S. 620 - 630. PHILADELPHIA: LIPPINCOTT WILLIAMS & WILKINS. ISSN 1540-0514

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Abstract

Background: Knowledge about the neuroinflammatory state during months after sudden cardiac arrest is scarce. Neuroinflammation is mediated by cells that express the 18 kDa translocator protein (TSPO). We determined the time course of TSPO-expressing cells in a rat model of sudden cardiac arrest using longitudinal in vivo positron emission tomography (PET) imaging with the TSPO-specific tracer [F-18]DAA1106 over a period of 6 months. Methods: Five male Sprague Dawley rats were resuscitated from 6 min sudden cardiac arrest due to ventricular fibrillation, three animals served as shams. PET measurements were performed on day 5, 8, 14, 90, and 180 after intervention. Magnetic resonance imaging was performed on day 140. Imaging was preceded by Barnes Maze spatial memory testing on day 3, 13, 90, and 180. Specificity of [F-18]DAA1106 binding was confirmed by Iba-1 immunohistochemistry. Results: [F-18]DAA1106 accumulated bilaterally in the dorsal hippocampus of all sudden cardiac arrest animals on all measured time points. Immunohistochemistry confirmed Iba-1 expressing cells in the hippocampal CA1 region. The number of Iba-1-immunoreactive objects per mm(2) was significantly correlated with [F-18]DAA1106 uptake. Additionally, two of the five sudden cardiac arrest rats showed bilateral TSPO-expression in the striatum that persisted until day 180. In Barnes Maze, the relative time spent in the target quadrant negatively correlates with dorsal hippocampal [F-18]DAA1106 uptake on day 14 and 180. Conclusions: After sudden cardiac arrest, TSPO remains expressed over the long-term. Sustainable treatment options for neuroinflammation may be considered to improve cognitive functions after sudden cardiac arrest.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schroeder, Daniel C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Popp, Erik UNSPECIFIED UNSPECIFIED UNSPECIFIED Rohleder, Cathrin UNSPECIFIED orcid.org/0000-0002-3559-1846 UNSPECIFIED Vus, Stefanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Bethencourt, David de la Puente UNSPECIFIED UNSPECIFIED UNSPECIFIED Finke, Simon R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zlatopolskiy, Boris D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zischler, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Drzezga, Alexander UNSPECIFIED orcid.org/0000-0001-6018-716X UNSPECIFIED Herff, Holger UNSPECIFIED UNSPECIFIED UNSPECIFIED Annecke, Thorsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Hucho, Tim UNSPECIFIED orcid.org/0000-0002-4147-9308 UNSPECIFIED Neumaier, Bernd UNSPECIFIED UNSPECIFIED UNSPECIFIED Boettiger, Bernd W. UNSPECIFIED UNSPECIFIED UNSPECIFIED Endepols, Heike UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-587249
DOI:	10.1097/SHK.0000000000001546
Journal or Publication Title:	Shock
Volume:	55
Number:	5
Page Range:	S. 620 - 630
Date:	2021
Publisher:	LIPPINCOTT WILLIAMS & WILKINS
Place of Publication:	PHILADELPHIA
ISSN:	1540-0514
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PERIPHERAL BENZODIAZEPINE-RECEPTORS; EUROPEAN-RESUSCITATION-COUNCIL; GLOBAL CEREBRAL-ISCHEMIA; ACTIVATED MICROGLIA; ANIMAL-MODELS; INTRACEREBROVENTRICULAR APPLICATION; CARDIOPULMONARY-RESUSCITATION; FOREBRAIN ISCHEMIA; BINDING-SITES; CELL-DAMAGE Multiple languages Critical Care Medicine; Hematology; Surgery; Peripheral Vascular Disease Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58724