Universität zu Köln

Eberhardt, Kirsten Alexandra, Dewald, Felix, Heger, Eva, Gieselmann, Lutz, Vanshylla, Kanika ORCID: 0000-0003-4552-9170, Wirtz, Maike, Kleipass, Franziska, Johannis, Wibke, Schommers, Philipp ORCID: 0000-0003-3375-6800, Gruell, Henning, Brensing, Karl August, Mueller, Roman-Ulrich, Augustin, Max ORCID: 0000-0002-2300-9337, Lehmann, Clara, Koch, Manuel ORCID: 0000-0002-2962-7814, Klein, Florian and Di Cristanziano, Veronica (2021). Evaluation of a New Spike (S)-Protein-Based Commercial Immunoassay for the Detection of Anti-SARS-CoV-2 IgG. Microorganisms, 9 (4). BASEL: MDPI. ISSN 2076-2607

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Abstract

Background: The investigation of the antibody response to SARS-CoV-2 represents a key aspect in facing the COVID-19 pandemic. In the present study, we compared the new Immundiagnostik IDK (R) anti-SARS-CoV-2 S1 IgG assay with four widely-used commercial serological assays for the detection of antibodies targeting S (spike) and NC (nucleocapsid) proteins. Methods: Serum samples were taken from an unbiased group of convalescent patients and from a negative control group. Sample were simultaneously analyzed by the new Immundiagnostik IDK(R) anti-SARS-CoV-2 S1 IgG assay, by the DiaSorin LIAISON(R) SARS-CoV-2 S1/S2 IgG assay, and by the Euroimmun anti-SARS-CoV-2 S1 IgG ELISA. Antibodies binding NC were detected by the Abbott SARS-CoV-2 IgG assay and by the pan-immunoglobulin immunoassay Roche Elecsys(R) anti-SARS-CoV-2. Moreover, we investigated samples of a group of COVID-19 convalescent subjects that were primarily tested S1 IgG non-reactive. Samples were also tested by live virus and pseudovirus neutralization tests. Results: Overall, the IDK (R) anti-SARS-CoV-2 S1 IgG assay showed the highest sensitivity among the evaluated spike (S) protein-based assays. Additionally, the Immundiagnostik assay correlated well with serum-neutralizing activity. Conclusions: The novel IDK(R) anti-SARS-CoV-2 S1 IgG assay showed high sensitivity and specificity, representing a valid option for use in the routine diagnostic.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Eberhardt, Kirsten Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Dewald, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Heger, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Gieselmann, Lutz UNSPECIFIED UNSPECIFIED UNSPECIFIED Vanshylla, Kanika UNSPECIFIED orcid.org/0000-0003-4552-9170 UNSPECIFIED Wirtz, Maike UNSPECIFIED UNSPECIFIED UNSPECIFIED Kleipass, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Johannis, Wibke UNSPECIFIED UNSPECIFIED UNSPECIFIED Schommers, Philipp UNSPECIFIED orcid.org/0000-0003-3375-6800 UNSPECIFIED Gruell, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Brensing, Karl August UNSPECIFIED UNSPECIFIED UNSPECIFIED Mueller, Roman-Ulrich UNSPECIFIED UNSPECIFIED UNSPECIFIED Augustin, Max UNSPECIFIED orcid.org/0000-0002-2300-9337 UNSPECIFIED Lehmann, Clara UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Klein, Florian UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Cristanziano, Veronica UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-589186
DOI:	10.3390/microorganisms9040733
Journal or Publication Title:	Microorganisms
Volume:	9
Number:	4
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2076-2607
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language NEUTRALIZING ANTIBODIES; SARS-COV-2 INFECTION; CLINICAL-EVALUATION; SEROPREVALENCE; PERFORMANCE Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58918