Universität zu Köln

Wang, Wanxue ORCID: 0000-0003-0606-6628, Salama, Mahmoud ORCID: 0000-0002-0282-8018, Todorov, Plamen, Spitkovsky, Dimirtry, Isachenko, Evgenia, Bongaarts, Rico, Rahimi, Gohar, Mallmann, Peter, Sukhikh, Gennady and Isachenko, Vladimir ORCID: 0000-0002-3674-543X (2021). New method of FACS analyzing and sorting of intact whole ovarian fragments (COPAS) after long time (24 h) cooling to 5 degrees C before cryopreservation. Cell Tissue Banking, 22 (3). S. 487 - 499. DORDRECHT: SPRINGER. ISSN 1573-6814

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Abstract

As recently announced by the American Society for Reproductive Medicine (ASRM), human ovarian tissue cryopreservation is an established option for fertility preservation in prepubertal girls and young women undergoing gonadotoxic treatments for cancer as well as some autoimmune diseases. Proper ovarian tissue assessment before and after cryopreservation is essential to increase success rates. Ovarian fragments from 16 patients were divided into small pieces in form of cortex with medulla, and randomly divided into the following two groups. Pieces of Group 1 (n = 16) were frozen immediately after operation, thawed and just after thawing their quality was analyzed. Group 2 pieces (n = 16) after operation were cooled to 5 degrees C for 24 h, then frozen after 24 h pre-cooling to 5 degrees C, thawed and just after thawing their quality was analyzed. The effectiveness of the pre-freezing cooling of tissue was evaluated by the development and viability of follicles (Calcein-AM and Propidium Iodide) using complex object parametric analyzer and sorter machine (COPAS). Positive effect of cooling of cells to low supra-zero temperatures on their future development after re-warming has been observed. New flow cytometry- technique is suitable for the evaluation and sorting of cryopreserved whole human whole intact ovarian fragments. Long time (24 h) cooling of ovarian tissue to 5 degrees C before cryopreservation has a trend of a cell viability increasing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Wanxue UNSPECIFIED orcid.org/0000-0003-0606-6628 UNSPECIFIED Salama, Mahmoud UNSPECIFIED orcid.org/0000-0002-0282-8018 UNSPECIFIED Todorov, Plamen UNSPECIFIED UNSPECIFIED UNSPECIFIED Spitkovsky, Dimirtry UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Evgenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Bongaarts, Rico UNSPECIFIED UNSPECIFIED UNSPECIFIED Rahimi, Gohar UNSPECIFIED UNSPECIFIED UNSPECIFIED Mallmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Sukhikh, Gennady UNSPECIFIED UNSPECIFIED UNSPECIFIED Isachenko, Vladimir UNSPECIFIED orcid.org/0000-0002-3674-543X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-590241
DOI:	10.1007/s10561-020-09898-1
Journal or Publication Title:	Cell Tissue Banking
Volume:	22
Number:	3
Page Range:	S. 487 - 499
Date:	2021
Publisher:	SPRINGER
Place of Publication:	DORDRECHT
ISSN:	1573-6814
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Cell Biology; Engineering, Biomedical Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59024