Uecal, Muammer, Maurer, Christa, Etschmaier, Vanessa, Hamberger, Daniel, Gruenbacher, Gerda, Toegl, Lennart, Roosen, Marvin J., Molcanyi, Marek, Vorholt, Daniela, Hatay, F. Fulya, Hescheler, Juergen, Pallasch, Christian, Schaefer, Ute and Patz, Silke (2021). Inflammatory Pre-Conditioning of Adipose-Derived Stem Cells with Cerebrospinal Fluid from Traumatic Brain Injury Patients Alters the Immunomodulatory Potential of ADSC Secretomes. J. Neurotrauma, 38 (16). S. 2311 - 2323. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1557-9042

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Abstract

Immunomodulation by adipose-tissue-derived stem cells (ADSCs) is of special interest for the alleviation of damaging inflammatory responses in central nervous system injuries. The present study explored the effects of cerebrospinal fluid (CSF) from traumatic brain injury (TBI) patients on this immunomodulatory potential of ADSCs. CSF conditioning of ADSCs increased messenger RNA levels of both pro- and anti-inflammatory genes compared to controls. Exposure of phorbol-12-myristate-13-acetate-differentiated THP1 macrophages to the secretome of CSF-conditioned ADSCs downregulated both proinflammatory (cyclooxygenase-2, tumor necrosis factor alpha) and anti-inflammatory (suppressor of cytokine signaling 3, interleukin-1 receptor antagonist, and transforming growth factor beta) genes in these cells. Interleukin-10 expression was elevated in both naive and conditioned secretomes. ADSC secretome treatment, further, induced macrophage maturation of THP1 cells and increased the percentage of CD11b(+), CD14(+), CD86(+), and, to a lesser extent, CD206(+) cells. This, moreover, enhanced the phagocytic activity of CD14(+) and CD86(+) cells, though independently of pre-conditioning. Secretome exposure, finally, also induced a reduction in the percentage of CD192(+) adherent cells in cultures of peripheral blood mononuclear cells (PBMCs) from both healthy subjects and TBI patients. This limited efficacy (of both naive and pre-conditioned secretomes) suggests that the effects of lymphocyte-monocyte paracrine signaling on the fate of cultured PBMCs are strongest upon adherent cell populations.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Uecal, MuammerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Maurer, ChristaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Etschmaier, VanessaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hamberger, DanielUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gruenbacher, GerdaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Toegl, LennartUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Roosen, Marvin J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Molcanyi, MarekUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Vorholt, DanielaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hatay, F. FulyaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hescheler, JuergenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pallasch, ChristianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schaefer, UteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Patz, SilkeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-592843
DOI: 10.1089/neu.2020.7017
Journal or Publication Title: J. Neurotrauma
Volume: 38
Number: 16
Page Range: S. 2311 - 2323
Date: 2021
Publisher: MARY ANN LIEBERT, INC
Place of Publication: NEW ROCHELLE
ISSN: 1557-9042
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
Critical Care Medicine; Clinical Neurology; NeurosciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59284

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