Bi, Guozhi, Su, Min, Li, Nan, Liang, Yu, Dang, Song, Xu, Jiachao, Hu, Meijuan ORCID: 0000-0001-5326-0816, Wang, Jizong ORCID: 0000-0003-1085-5347, Zou, Minxia, Deng, Yanan ORCID: 0000-0002-1383-4368, Li, Qiyu, Huang, Shijia, Li, Jiejie, Chai, Jijie, He, Kangmin, Chen, Yu-hang and Zhou, Jian-Min (2021). The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling. Cell, 184 (13). S. 3528 - 3554. CAMBRIDGE: CELL PRESS. ISSN 1097-4172

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Abstract

Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in plants and animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane, The ZAR1 resistosome displayed ion channel activity in Xenopus oocytes in a manner dependent on a conserved acidic residue Glu11 situated in the channel pore. Pre-assembled ZAR1 resistosome was readily incorporated into planar lipid-bilayers and displayed calcium-permeable cation-selective channel activity. Furthermore, we show that activation of ZAR1 in the plant cell led to Glu11 -dependent Ca2+ influx, perturbation of subcellular structures, production of reactive oxygen species, and cell death. The results thus support that the ZAR1 resisto-some acts as a calcium-permeable cation channel to trigger immunity and cell death.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Bi, GuozhiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Su, MinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Li, NanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liang, YuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dang, SongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Xu, JiachaoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hu, MeijuanUNSPECIFIEDorcid.org/0000-0001-5326-0816UNSPECIFIED
Wang, JizongUNSPECIFIEDorcid.org/0000-0003-1085-5347UNSPECIFIED
Zou, MinxiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Deng, YananUNSPECIFIEDorcid.org/0000-0002-1383-4368UNSPECIFIED
Li, QiyuUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Huang, ShijiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Li, JiejieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chai, JijieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
He, KangminUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chen, Yu-hangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Zhou, Jian-MinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-596586
DOI: 10.1016/j.cell.2021.05.003
Journal or Publication Title: Cell
Volume: 184
Number: 13
Page Range: S. 3528 - 3554
Date: 2021
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 1097-4172
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
SINGLE-MOLECULE ANALYSIS; DISEASE RESISTANCE GENE; PLASMA-MEMBRANE; OXIDATIVE BURST; ARABIDOPSIS; EFFECTOR; DYNAMICS; PROTEIN; PSEUDOKINASE; RECOGNITIONMultiple languages
Biochemistry & Molecular Biology; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59658

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