Universität zu Köln

Blanc, Melanie, Antczak, Philipp, Cousin, Xavier, Grunau, Christoph, Scherbak, Nikolai, Ruegg, Joelle and Keiter, Steffen H. (2021). The insecticide permethrin induces transgenerational behavioral changes linked to transcriptomic and epigenetic alterations in zebrafish (Danio rerio). Sci. Total Environ., 779. AMSTERDAM: ELSEVIER. ISSN 1879-1026

Full text not available from this repository.

Abstract

The pyrethroid insecticide permethrin is widely used for agricultural and domestic purposes. Previous data indicated that it acts as a developmental neurotoxicant and can induce transgenerational effects in non target organisms. However, associated underlying mechanisms remain unclear. The aim of this study was to investigate permethrin-related transgenerational effects in the zebrafish model, and to identify possible molecular mechanisms underlying inheritance. Zebrafish (F 0) were exposed to permethrin during early life (2 h post-fertilization up to 28 days). The F1 and F2 offspring generations were obtained by pairing exposed F0 males and females, and were bred unexposed. Locomotor and anxiety behavior were investigated, together with transcriptomic and epigenomic (DNA methylation) changes in brains. Permethrin exposed F0 fish were hypoactive at adulthood, while males from the F1 and F2 generations showed a specific decrease in anxiety-like behavior. In F0, transcriptomic data showed enrichment in pathways related to glutamatergic synapse activity, which may partly underlie the behavioral effects. In F1 and F2 males, dysregulation of similar pathways was observed, including a subset of differentially methylated regions that were inherited from the F0 to the F2 generation and indicated stable dysregulation of glutamatergic signaling. Altogether, the present results provide novel evidence on the transgenerational neurotoxic effects of permethrin, as well as mechanistic insight: a transient exposure induces persistent transcriptional and DNA methylation changes that may translate into transgenerational alteration of glutamatergic signaling and, thus, into behavioral alterations. (c) 2021 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Blanc, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Antczak, Philipp UNSPECIFIED UNSPECIFIED UNSPECIFIED Cousin, Xavier UNSPECIFIED UNSPECIFIED UNSPECIFIED Grunau, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Scherbak, Nikolai UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruegg, Joelle UNSPECIFIED UNSPECIFIED UNSPECIFIED Keiter, Steffen H. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-598241
DOI:	10.1016/j.scitotenv.2021.146404
Journal or Publication Title:	Sci. Total Environ.
Volume:	779
Date:	2021
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1879-1026
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language EARLY-LIFE EXPOSURE; DNA METHYLATION; PYRETHROID INSECTICIDES; DEVELOPMENTAL EXPOSURE; PRENATAL EXPOSURE; GENE-EXPRESSION; MESSENGER-RNA; IN-SILICO; INHERITANCE; VIMENTIN Multiple languages Environmental Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59824