Universität zu Köln

Blohm, Martin, Hahn, Andreas ORCID: 0000-0002-5157-8369, Hagen, Ralf Matthias ORCID: 0000-0003-4875-1519, Eberhardt, Kirsten Alexandra, Rohde, Holger, Leboulle, Gerard, Feldt, Torsten, Sarfo, Fred Stephen, Di Cristanziano, Veronica, Frickmann, Hagen and Loderstaedt, Ulrike (2021). Comparison of Two Real-Time PCR Assays Targeting Ribosomal Sequences for the Identification of Cystoisospora belli in Human Stool Samples. Pathogens, 10 (8). BASEL: MDPI. ISSN 2076-0817

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Abstract

Cystoisospora (C.) belli is a coccidian parasite associated with acute or chronic gastroenteritis in immunocompromised patients. Dissatisfactory sensitivity of microscopy as the diagnostic standard approach has been described. Here, we comparatively evaluated two real-time PCRs targeting ribosomal RNA gene sequences of C. belli in stool in a test comparison without a reference standard applying latent class analysis. Therefore, 1000 stool samples from Ghanaian HIV (human immunodeficiency virus) patients (n = 905) as well as military returnees from the tropics (n = 95) were assessed by both assays in parallel. After the exclusion of 33 samples showing PCR inhibition, 29 and 33 positive results were recorded with the 5.8S rRNA gene/ITS-2 sequence PCR and the ITS-2 sequence PCR, respectively, resulting in an accuracy-adjusted prevalence of 3.2%. Nearly perfect agreement between both assays was indicated by Fleiss' kappa of 0.933 with sensitivity and specificity of 92.8% and 100% as well as 100% and 99.8% for the 5.8S rRNA gene/ITS-2 sequence PCR and the ITS-2 sequence PCR, respectively. Both assays proved to be suitable for the diagnosis of C. belli in human stool samples with slightly better sensitivity of the ITS-2 sequence assay, while the 5.8S rRNA gene/ITS-2 sequence PCR may be considered for confirmatory testing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Blohm, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Hahn, Andreas UNSPECIFIED orcid.org/0000-0002-5157-8369 UNSPECIFIED Hagen, Ralf Matthias UNSPECIFIED orcid.org/0000-0003-4875-1519 UNSPECIFIED Eberhardt, Kirsten Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Rohde, Holger UNSPECIFIED UNSPECIFIED UNSPECIFIED Leboulle, Gerard UNSPECIFIED UNSPECIFIED UNSPECIFIED Feldt, Torsten UNSPECIFIED UNSPECIFIED UNSPECIFIED Sarfo, Fred Stephen UNSPECIFIED UNSPECIFIED UNSPECIFIED Di Cristanziano, Veronica UNSPECIFIED UNSPECIFIED UNSPECIFIED Frickmann, Hagen UNSPECIFIED UNSPECIFIED UNSPECIFIED Loderstaedt, Ulrike UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-599918
DOI:	10.3390/pathogens10081053
Journal or Publication Title:	Pathogens
Volume:	10
Number:	8
Date:	2021
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2076-0817
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language POLYMERASE-CHAIN-REACTION; INTESTINAL PARASITES; PROTOZOAN OOCYSTS; ISOSPORA-BELLI; FECAL SAMPLES; INFECTIONS; DIAGNOSIS; MICROSPORIDIA; PREVALENCE; CYCLOSPORA Multiple languages Microbiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59991