Universität zu Köln

Jachimowicz, Ron Daniel, Pieper, Luise, Reinke, Sarah, Gontarewicz, Artur, Pluetschow, Annette, Haverkamp, Heinz, Frauenfeld, Leonie, Fend, Falko, Overkamp, Mathis, Jochims, Franziska, Thorns, Christoph, Hansmann, Martin Leo, Moller, Peter, Rosenwald, Andreas, Stein, Harald, Reinhardt, Hans Christian, Borchmann, Peter, von Tresckow, Bastian, Engert, Andreas and Klapper, Wolfram (2021). Whole-slide image analysis of the tumor as a predictor of adverse outcome in patients with advanced-stage classical Hodgkin lymphoma treated with BEACOPP. Haematologica, 106 (6). S. 1684 - 1693. PAVIA: FERRATA STORTI FOUNDATION. ISSN 0390-6078

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Abstract

A subset of patients with advanced-stage classical Hodgkin lymphoma (cHL) relapse or progress following standard treatment. Given their dismal prognosis, identifying this group of patients upfront represents an important medical need. While prior research has identified characteristics of the tumor microenvironment, which are associated with cHL outcomes, biomarkers that are developed and validated in this high-risk group are still lacking. Here, we applied whole-slide image analysis (WSI), a quantitative, large-scale assessment of tumor composition that utilizes conventional histopathology slides. We conducted WSI on pre-treatment biopsies from 340 patients with advanced-stage cHL enrolled in the HD12 and HD15 trials of the German Hodgkin Study Group (GHSG), and tested our results in a validation cohort of 147 advanced-stage cHL patients within the GHSG HD18 trial. All patients were treated with BEACOPP-based regimens. By quantifying T cells, B cells, Hodgkin and Reed-Sternberg cells and macrophages with WSI, 80% of all cells in the tumor tissue were identified. Crucially, low B-cell count was associated with significantly reduced progression-free survival and overall survival, while the content of T cells, macrophages and Hodgkin and Reed-Sternberg cells was not associated with the risk of progression or relapse in the study cohort. We further validated low B-cell content as a prognostic factor for progression-free survival and overall survival in the validation cohort and demonstrated the good inter-observer agreement of WSI. WSI may represent a key tool for risk stratification of advanced-stage cHL and can easily be added to the standard diagnostic histopathology work-up.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Jachimowicz, Ron Daniel UNSPECIFIED UNSPECIFIED UNSPECIFIED Pieper, Luise UNSPECIFIED UNSPECIFIED UNSPECIFIED Reinke, Sarah UNSPECIFIED UNSPECIFIED UNSPECIFIED Gontarewicz, Artur UNSPECIFIED UNSPECIFIED UNSPECIFIED Pluetschow, Annette UNSPECIFIED UNSPECIFIED UNSPECIFIED Haverkamp, Heinz UNSPECIFIED UNSPECIFIED UNSPECIFIED Frauenfeld, Leonie UNSPECIFIED UNSPECIFIED UNSPECIFIED Fend, Falko UNSPECIFIED UNSPECIFIED UNSPECIFIED Overkamp, Mathis UNSPECIFIED UNSPECIFIED UNSPECIFIED Jochims, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Thorns, Christoph UNSPECIFIED UNSPECIFIED UNSPECIFIED Hansmann, Martin Leo UNSPECIFIED UNSPECIFIED UNSPECIFIED Moller, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED Rosenwald, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Stein, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Reinhardt, Hans Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Borchmann, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED von Tresckow, Bastian UNSPECIFIED UNSPECIFIED UNSPECIFIED Engert, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Klapper, Wolfram UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-600714
DOI:	10.3324/haematol.2019.243287
Journal or Publication Title:	Haematologica
Volume:	106
Number:	6
Page Range:	S. 1684 - 1693
Date:	2021
Publisher:	FERRATA STORTI FOUNDATION
Place of Publication:	PAVIA
ISSN:	0390-6078
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REED-STERNBERG CELLS; INTERNATIONAL PROGNOSTIC SCORE; CD20 EXPRESSION; B-CELLS; MACROPHAGES; SURVIVAL; CHEMOTHERAPY; MICROENVIRONMENT; CHECKPOINT; REVEALS Multiple languages Hematology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/60071