Gabriel, Elke, Albanna, Walid, Pasquini, Giovanni ORCID: 0000-0002-5750-6307, Ramani, Anand, Josipovic, Natasa ORCID: 0000-0001-7683-0869, Mariappan, Aruljothi, Schinzel, Friedrich, Karch, Celeste M., Bao, Guobin, Gottardo, Marco, Suren, Ata Alp, Hescheler, Jurgen, Nagel-Wolfrum, Kerstin, Persico, Veronica, Rizzoli, Silvio O., Altmuller, Janine, Riparbelli, Maria Giovanna, Callaini, Giuliano, Goureau, Olivier, Papantonis, Argyris, Busskamp, Volker, Schneider, Toni and Gopalakrishnan, Jay (2021). Human brain organoids assemble functionally integrated bilateral optic vesicles. Cell Stem Cell, 28 (10). S. 1740 - 1767. CAMBRIDGE: CELL PRESS. ISSN 1875-9777

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Abstract

During embryogenesis, optic vesicles develop from the diencephalon via a multistep process of organogenesis. Using induced pluripotent stem cell (iPSC)-derived human brain organoids, we attempted to simplify the complexities and demonstrate formation of forebrain-associated bilateral optic vesicles, cellular diversity, and functionality. Around day 30, brain organoids attempt to assemble optic vesicles, which develop progressively as visible structures within 60 days. These optic vesicle-containing brain organoids (OVB-organoids) constitute a developing optic vesicle's cellular components, including primitive corneal epithelial and lens-like cells, retinal pigment epithelia, retinal progenitor cells, axon-like projections, and electrically active neuronal networks. OVB-organoids also display synapsin-1, CTIP-positive myelinated cortical neurons, and microglia. Interestingly, various light intensities could trigger photosensitive activity of OVB-organoids, and light sensitivities could be reset after transient photobleaching. Thus, brain organoids have the intrinsic ability to self-organize forebrain-associated primitive sensory structures in a topographically restricted manner and can allow interorgan interaction studies within a single organoid.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Gabriel, ElkeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Albanna, WalidUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Pasquini, GiovanniUNSPECIFIEDorcid.org/0000-0002-5750-6307UNSPECIFIED
Ramani, AnandUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Josipovic, NatasaUNSPECIFIEDorcid.org/0000-0001-7683-0869UNSPECIFIED
Mariappan, AruljothiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schinzel, FriedrichUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Karch, Celeste M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bao, GuobinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gottardo, MarcoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Suren, Ata AlpUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hescheler, JurgenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nagel-Wolfrum, KerstinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Persico, VeronicaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rizzoli, Silvio O.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Altmuller, JanineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Riparbelli, Maria GiovannaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Callaini, GiulianoUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Goureau, OlivierUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Papantonis, ArgyrisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Busskamp, VolkerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schneider, ToniUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Gopalakrishnan, JayUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-600872
DOI: 10.1016/j.stem.2021.07.010
Journal or Publication Title: Cell Stem Cell
Volume: 28
Number: 10
Page Range: S. 1740 - 1767
Date: 2021
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 1875-9777
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
RETINAL-PIGMENT EPITHELIUM; PLURIPOTENT STEM-CELLS; HOMEOBOX GENE; PRENATAL DEVELOPMENT; GANGLIONIC EMINENCE; NEURAL RETINA; R PACKAGE; EXPRESSION; FOREBRAIN; GENERATIONMultiple languages
Cell & Tissue Engineering; Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/60087

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