Universität zu Köln

Naue, Jana, Pfeifer, Manuel, Augustin, Christa, Becker, Julia, Fleckhaus, Jan, Grabmueller, Melanie, Han, Yang, Heidorn, Frank, Hollaender, Olivia, Klein-Unseld, Rachel, Kulstein, Galina, Lichtenwald, Julia, Neubauer, Jacqueline, Suarez, Philippe, Haas, Cordula, Schneider, Peter M., Vennemann, Marielle and Boehme, Petra (2021). Forensic DNA methylation analysis Second technical collaborative exercise by the working group on molecular age estimation of the German Society of Legal Medicine. Rechtsmedizin, 31 (3). S. 202 - 217. NEW YORK: SPRINGER. ISSN 1434-5196

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Abstract

With the discovery of age-related epigenetic changes DNA methylation (DNAm) has shown new possibilities for the estimation of the age of an individual. The method has been intensively researched and its application in forensic casework is regulated by an amendment of </n> 81e of the German Code of Criminal Procedures (StPO). To investigate the degree of DNAm new techniques must be established and validated. This necessitates investigation of the comparability of measurement results from different forensic laboratories. In winter 2019/2020 the molecular age estimation working group of the German Society of Legal Medicine (DGRM) conducted a second proficiency test to investigate this comparability using minisequencing and pyrosequencing for quantitative analysis of DNAm. This was based on the experience from the first proficiency test in 2018/2019, the results of which are presented in this edition of the journal. The current study includes the results of DNAm analysis from 12 laboratories (in total 14 participating laboratories), some of which have used both methods. In addition, four laboratories performed an age estimation using their own marker combinations and models. As these are based on different reference data and marker combinations, no qualitative comparison of the models was done but the fundamental potential of the methodology was clarified. The aim of the interlaboratory comparison was to evaluate the comparability of the DNAm measurements and to assess possible influencing factors, such as the extraction method and the device used. The results showed that the measured DNAm values of the investigated markers can differ between minisequencing and pyrosequencing as well as within the respective method between laboratories, so that fluctuations are to be expected.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Naue, Jana UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfeifer, Manuel UNSPECIFIED UNSPECIFIED UNSPECIFIED Augustin, Christa UNSPECIFIED UNSPECIFIED UNSPECIFIED Becker, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Fleckhaus, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Grabmueller, Melanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Han, Yang UNSPECIFIED UNSPECIFIED UNSPECIFIED Heidorn, Frank UNSPECIFIED UNSPECIFIED UNSPECIFIED Hollaender, Olivia UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein-Unseld, Rachel UNSPECIFIED UNSPECIFIED UNSPECIFIED Kulstein, Galina UNSPECIFIED UNSPECIFIED UNSPECIFIED Lichtenwald, Julia UNSPECIFIED UNSPECIFIED UNSPECIFIED Neubauer, Jacqueline UNSPECIFIED UNSPECIFIED UNSPECIFIED Suarez, Philippe UNSPECIFIED UNSPECIFIED UNSPECIFIED Haas, Cordula UNSPECIFIED UNSPECIFIED UNSPECIFIED Schneider, Peter M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vennemann, Marielle UNSPECIFIED UNSPECIFIED UNSPECIFIED Boehme, Petra UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-603734
DOI:	10.1007/s00194-021-00493-6
Journal or Publication Title:	Rechtsmedizin
Volume:	31
Number:	3
Page Range:	S. 202 - 217
Date:	2021
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1434-5196
Language:	German
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PREDICTION; BLOOD Multiple languages Medicine, Legal Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/60373