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Mulcahy, Mulero, Victoriano, Muller, Sylviane, Munch, Christian, Munjal, Ashok, Munoz-Canoves, Pura, Munoz-Galdeano, Teresa ORCID: 0000-0003-3526-819X, Munz, Christian, Murakawa, Tomokazu, Muratori, Claudia, Murphy, Brona M., Murphy, J. Patrick, Murthy, Aditya, Myohanen, Timo T., Mysorekar, Indira U., Mytych, Jennifer ORCID: 0000-0002-1265-973X, Nabavi, Seyed Mohammad ORCID: 0000-0001-8859-5675, Nabissi, Massimo, Nagy, Peter ORCID: 0000-0002-5053-0646, Nah, Jihoon, Nahimana, Aimable, Nakagawa, Ichiro, Nakamura, Ken, Nakatogawa, Hitoshi, Nandi, Shyam S., Nanjundan, Meera, Nanni, Monica, Napolitano, Gennaro, Nardacci, Roberta ORCID: 0000-0002-9209-1207, Narita, Masashi, Nassif, Melissa ORCID: 0000-0002-5039-5245, Nathan, Ilana, Natsumeda, Manabu ORCID: 0000-0003-3098-8323, Naude, Ryno J., Naumann, Christin, Naveiras, Olaia ORCID: 0000-0003-3434-0022, Navid, Fatemeh, Nawrocki, Steffan T., Nazarko, Taras Y., Nazio, Francesca, Negoita, Florentina, Neill, Thomas, Neisch, Amanda L., Neri, Luca M., Netea, Mihai G., Neubert, Patrick, Neufeld, Thomas P., Neumann, Dietbert ORCID: 0000-0002-4316-684X, Neutzner, Albert, Newton, Phillip T., Ney, Paul A., Nezis, Ioannis P., Ng, Charlene C. W., Ng, Tzi Bun, Nguyen, Hang T. T., Nguyen, Long T., Ni, Hong-Min, Cheallaigh, Cliona Ni, Ni, Zhenhong, Nicolao, M. Celeste, Nicoli, Francesco ORCID: 0000-0001-6327-5958, Nieto-Diaz, Manuel ORCID: 0000-0002-7244-794X, Nilsson, Per, Ning, Shunbin, Niranjan, Rituraj, Nishimune, Hiroshi, Niso-Santano, Mireia, Nixon, Ralph A., Nobili, Annalisa ORCID: 0000-0001-8369-1561, Nobrega, Clevio, Noda, Takeshi ORCID: 0000-0003-3581-7961, Nogueira-Recalde, Uxia, Nolan, Trevor M., Nombela, Ivan, Novak, Ivana, Novoa, Beatriz, Nozawa, Takashi, Nukina, Nobuyuki, Nussbaum-Krammer, Carmen and Nylandsted, Jesper ORCID: 0000-0001-6474-5093 (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy, 17 (1). S. 1 - 383. PHILADELPHIA: TAYLOR & FRANCIS INC. ISSN 1554-8635

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Abstract

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
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Agam, GalilaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
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Aggarwal, Bharat B.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
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Negoita, FlorentinaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
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Nguyen, Long T.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ni, Hong-MinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cheallaigh, Cliona NiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ni, ZhenhongUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nicolao, M. CelesteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nicoli, FrancescoUNSPECIFIEDorcid.org/0000-0001-6327-5958UNSPECIFIED
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Nilsson, PerUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ning, ShunbinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
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Nishimune, HiroshiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niso-Santano, MireiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nixon, Ralph A.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nobili, AnnalisaUNSPECIFIEDorcid.org/0000-0001-8369-1561UNSPECIFIED
Nobrega, ClevioUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Noda, TakeshiUNSPECIFIEDorcid.org/0000-0003-3581-7961UNSPECIFIED
Nogueira-Recalde, UxiaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nolan, Trevor M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nombela, IvanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Novak, IvanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Novoa, BeatrizUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nozawa, TakashiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nukina, NobuyukiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nussbaum-Krammer, CarmenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Nylandsted, JesperUNSPECIFIEDorcid.org/0000-0001-6474-5093UNSPECIFIED
URN: urn:nbn:de:hbz:38-608549
DOI: 10.1080/15548627.2020.1797280
Journal or Publication Title: Autophagy
Volume: 17
Number: 1
Page Range: S. 1 - 383
Date: 2021
Publisher: TAYLOR & FRANCIS INC
Place of Publication: PHILADELPHIA
ISSN: 1554-8635
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CHAPERONE-MEDIATED AUTOPHAGY; ACTIVATED PROTEIN-KINASE; PROGRAMMED CELL-DEATH; STARVATION-INDUCED AUTOPHAGY; ENDOPLASMIC-RETICULUM STRESS; NF-KAPPA-B; BREAST-CANCER CELLS; LIFE-SPAN EXTENSION; AMYOTROPHIC-LATERAL-SCLEROSIS; GENOME-WIDE ASSOCIATIONMultiple languages
Cell BiologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/60854

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