Bornewasser, Lisa ORCID: 0000-0002-3890-107X, Domnick, Christof and Kath-Schorr, Stephanie (2022). Stronger together for in-cell translation: natural and unnatural base modified mRNA. Chem. Sci., 13 (17). S. 4753 - 4763. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 2041-6539
Full text not available from this repository.Abstract
The preparation of highly modified mRNAs and visualization of their cellular distribution are challenging. We report in-cell application of in vitro transcribed mRNA containing natural base modifications and site-specifically introduced artificial nucleotides. Click chemistry on mRNA allows visualization in cells with excellent signal intensities. While non-specific introduction of reporter groups often leads to loss in mRNA functionality, we combined the benefits from site-specificity in the 3 '-UTR incorporated unnatural nucleotides with the improved translation efficiency of the natural base modifications psi and 5mC. A series of experiments is described to observe, quantify and verify mRNA functionality. This approach represents a new way to visualize mRNA delivery into cells and monitor its spread on a cellular level and translation efficiency. We observed increased protein expression from this twofold chemically modified, artificial mRNA counterbalancing a reduced transfection rate. This synergetic effect can be exploited as a powerful tool for future research on mRNA therapeutics.
Item Type: | Journal Article | ||||||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-658195 | ||||||||||||||||
DOI: | 10.1039/d2sc00670g | ||||||||||||||||
Journal or Publication Title: | Chem. Sci. | ||||||||||||||||
Volume: | 13 | ||||||||||||||||
Number: | 17 | ||||||||||||||||
Page Range: | S. 4753 - 4763 | ||||||||||||||||
Date: | 2022 | ||||||||||||||||
Publisher: | ROYAL SOC CHEMISTRY | ||||||||||||||||
Place of Publication: | CAMBRIDGE | ||||||||||||||||
ISSN: | 2041-6539 | ||||||||||||||||
Language: | English | ||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||
Subjects: | no entry | ||||||||||||||||
Uncontrolled Keywords: |
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URI: | http://kups.ub.uni-koeln.de/id/eprint/65819 |
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