Universität zu Köln

Hussainy, Mohammad ORCID: 0000-0002-4849-8894, Korsching, Sigrun, I and Tresch, Achim (2022). Pseudotime analysis reveals novel regulatory factors for multigenic onset and monogenic transition of odorant receptor expression. Sci Rep, 12 (1). BERLIN: NATURE PORTFOLIO. ISSN 2045-2322

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Abstract

During their maturation from horizontal basal stem cells, olfactory sensory neurons (OSNs) are known to select exactly one out of hundreds of olfactory receptors (ORs) and express it on their surface, a process called monogenic selection. Monogenic expression is preceded by a multigenic phase during which several OR genes are expressed in a single OSN. Here, we perform pseudotime analysis of a single cell RNA-Seq dataset of murine olfactory epithelium to precisely align the multigenic and monogenic expression phases with the cell types occurring during OSN differentiation. In combination with motif analysis of OR gene cluster-associated enhancer regions, we identify known and novel transcription (co-)factors (Ebf1, Lhx2, Ldb1, Fos and Ssbp2) and chromatin remodelers (Kdm1a, Eed and Zmynd8) associated with OR expression. The inferred temporal order of their activity suggests novel mechanisms contributing to multigenic OR expression and monogenic selection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Hussainy, Mohammad UNSPECIFIED orcid.org/0000-0002-4849-8894 UNSPECIFIED Korsching, Sigrun, I UNSPECIFIED UNSPECIFIED UNSPECIFIED Tresch, Achim UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-658788
DOI:	10.1038/s41598-022-20106-w
Journal or Publication Title:	Sci Rep
Volume:	12
Number:	1
Date:	2022
Publisher:	NATURE PORTFOLIO
Place of Publication:	BERLIN
ISSN:	2045-2322
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GLOBOSE BASAL-CELLS; POLYCOMB REPRESSIVE COMPLEX; OLFACTORY SENSORY NEURONS; DEMETHYLASE-1 LSD1; GENE-EXPRESSION; EPITHELIUM; PROMOTER; GLOMERULI; MECHANISM; ENHANCER Multiple languages Multidisciplinary Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/65878