Universität zu Köln

Mattern, Annabelle, Classen, Rebecca, Wolf, Annemarie, Pouokam, Ervice, Schlueter, Klaus-Dieter, Wickleder, Mathias S. and Diener, Martin (2022). Multivalent stimulation of beta(1)-, but not beta(2)-receptors by adrenaline functionalised gold nanoparticles. Nanoscale Adv., 4 (15). S. 3182 - 3194. CAMBRIDGE: ROYAL SOC CHEMISTRY. ISSN 2516-0230

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Abstract

In this study, we present a strategy for the synthesis of catecholamine functionalised gold nanoparticles and investigated their multivalent interactions with adrenergic receptors in different biological systems. The catecholamines adrenaline and noradrenaline represent key examples of adrenergic agonists. We used gold nanoparticles as carriers and functionalised them on their surface with a variety of these neurotransmitter molecules. For this purpose, we synthesised each ligand separately using mercaptoundecanoic acid as a bifunctional linking unit and adrenaline (or noradrenaline) as a biogenic amine. This ligand was then immobilised onto the surface of presynthesised spherical monodispersive gold nanoparticles in a ligand exchange reaction. After detailed analytical characterisations, the functionalised gold nanoparticles were investigated for their interactions with adrenergic receptors in intestinal, cardiac and respiratory tissues. Whereas the contractility of respiratory smooth muscle cells (regulated by beta(2)-receptors) was not influenced, (nor)adrenaline functionalised nanoparticles administered in nanomolar concentrations induced epithelial K+ secretion (mediated via different beta-receptors) and increased contractility of isolated rat cardiomyocytes (mediated by beta(1)-receptors). The present results suggest differences in the accessibility of adrenergic agonists bound to gold nanoparticles to the binding pockets of different beta-receptor subtypes.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Mattern, Annabelle UNSPECIFIED UNSPECIFIED UNSPECIFIED Classen, Rebecca UNSPECIFIED UNSPECIFIED UNSPECIFIED Wolf, Annemarie UNSPECIFIED UNSPECIFIED UNSPECIFIED Pouokam, Ervice UNSPECIFIED UNSPECIFIED UNSPECIFIED Schlueter, Klaus-Dieter UNSPECIFIED UNSPECIFIED UNSPECIFIED Wickleder, Mathias S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Diener, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-665463
DOI:	10.1039/d2na00171c
Journal or Publication Title:	Nanoscale Adv.
Volume:	4
Number:	15
Page Range:	S. 3182 - 3194
Date:	2022
Publisher:	ROYAL SOC CHEMISTRY
Place of Publication:	CAMBRIDGE
ISSN:	2516-0230
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language VIVO PHOTODYNAMIC THERAPY; CATECHOL OXIDATION; DRUG-DELIVERY; ACTIVATION; NUCLEATION; ANTIBODY; BINDING; SURFACE Multiple languages Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66546