Universität zu Köln

Klein, Silvan ORCID: 0000-0003-0113-5137, Siegmund, Andreas, Eigenberger, Andreas ORCID: 0000-0002-2141-8786, Hartmann, Valerie, Langewost, Felix, Hammer, Nicolas, Anker, Alexandra, Klein, Konstantin, Morsczeck, Christian, Prantl, Lukas ORCID: 0000-0003-2454-2499 and Felthaus, Oliver (2022). Peripheral Nerve Regeneration-Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion. Cells, 11 (18). BASEL: MDPI. ISSN 2073-4409

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Abstract

The lack of supportive Schwann cells in segmental nerve lesions seems to be one cornerstone for the problem of insufficient nerve regeneration. Lately, adipose-tissue-derived stem cells (ASCs) differentiated towards SC (Schwann cell)-like cells seem to fulfill some of the needs for ameliorated nerve recovery. In this study, three differentiation protocols were investigated for their ability to differentiate ASCs from rats into specialized SC phenotypes. The differentiated ASCs (dASCs) were compared for their expressions of neurotrophins (NGF, GDNF, BDNF), myelin markers (MBP, P0), as well as glial-marker proteins (S100, GFAP) by RT-PCR, ELISA, and Western blot. Additionally, the influence of the medium conditioned by dASCs on a neuron-like cell line was evaluated. The dASCs were highly diverse in their expression profiles. One protocol yielded relatively high expression rates of neurotrophins, whereas another protocol induced myelin-marker expression. These results were reproducible when the ASCs were differentiated on surfaces potentially used for nerve guidance conduits. The NGF secretion affected the neurite outgrowth significantly. It remains uncertain what features of these SC-like cells contribute the most to adequate functional recovery during the different phases of nerve recovery. Nevertheless, therapeutic applications should consider these diverse phenotypes as a potential approach for stem-cell-based nerve-injury treatment.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Klein, Silvan UNSPECIFIED orcid.org/0000-0003-0113-5137 UNSPECIFIED Siegmund, Andreas UNSPECIFIED UNSPECIFIED UNSPECIFIED Eigenberger, Andreas UNSPECIFIED orcid.org/0000-0002-2141-8786 UNSPECIFIED Hartmann, Valerie UNSPECIFIED UNSPECIFIED UNSPECIFIED Langewost, Felix UNSPECIFIED UNSPECIFIED UNSPECIFIED Hammer, Nicolas UNSPECIFIED UNSPECIFIED UNSPECIFIED Anker, Alexandra UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Konstantin UNSPECIFIED UNSPECIFIED UNSPECIFIED Morsczeck, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Prantl, Lukas UNSPECIFIED orcid.org/0000-0003-2454-2499 UNSPECIFIED Felthaus, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-665751
DOI:	10.3390/cells11182887
Journal or Publication Title:	Cells
Volume:	11
Number:	18
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4409
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language REPROGRAMS SCHWANN-CELLS; MARROW STROMAL CELLS; NEUROTROPHIC FACTORS; FUNCTIONAL RECOVERY; RECEPTOR; LAMININ; EXPRESSION; PHENOTYPE; OUTGROWTH; NEURONS Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66575