Universität zu Köln

Boyan, George and Ehrhardt, Erica ORCID: 0000-0002-9252-1414 (2022). Early embryonic development of Johnston's organ in the antenna of the desert locust Schistocerca gregaria. Dev. Genes Evol., 232 (5-6). S. 103 - 114. NEW YORK: SPRINGER. ISSN 1432-041X

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Abstract

Johnston's organ has been shown to act as an antennal auditory organ across a spectrum of insect species. In the hemimetabolous desert locust Schistocerca gregaria, Johnston's organ must be functional on hatching and so develops in the pedicellar segment of the antenna during embryogenesis. Here, we employ the epithelial cell marker Lachesin to identify the pedicellar domain of the early embryonic antenna and then triple-label against Lachesin, the mitosis marker phosphohistone-3, and neuron-specific horseradish peroxidase to reveal the sense-organ precursors for Johnston's organ and their lineages. Beginning with a single progenitor at approximately a third of embryogenesis, additional precursors subsequently appear in both the ventral and dorsal pedicellar domains, each generating a lineage or clone. Lineage locations are remarkably conserved across preparations and ages, consistent with the epithelium possessing an underlying topographic coordinate system that determines the cellular organization of Johnston's organ. By mid-embryogenesis, twelve lineages are arranged circumferentially in the pedicel as in the adult structure. Each sense-organ precursor is associated with a smaller mitotically active cell from which the neuronal complement of each clone may derive. Neuron numbers within a clone increase in discrete steps with age and are invariant between clones and across preparations of a given age. At mid-embryogenesis, each clone comprises five cells consolidated into a tightly bound cartridge. A long scolopale extends apically from each cartridge to an insertion point in the epithelium, and bundled axons project basally toward the brain. Comparative data suggest mechanisms that might also regulate the developmental program of Johnston's organ in the locust.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Boyan, George UNSPECIFIED UNSPECIFIED UNSPECIFIED Ehrhardt, Erica UNSPECIFIED orcid.org/0000-0002-9252-1414 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-667273
DOI:	10.1007/s00427-022-00695-2
Journal or Publication Title:	Dev. Genes Evol.
Volume:	232
Number:	5-6
Page Range:	S. 103 - 114
Date:	2022
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-041X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TELEOGRYLLUS-COMMODUS WALKER; PERIPHERAL NERVOUS-SYSTEM; CHORDOTONAL ORGANS; PIONEER NEURONS; AUDITORY-SYSTEM; PRONEURAL GENE; FINE-STRUCTURE; CELL LINEAGE; DROSOPHILA; GRASSHOPPER Multiple languages Cell Biology; Evolutionary Biology; Developmental Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66727