Universität zu Köln

Gehling, Kristin ORCID: 0000-0002-7933-3777, Parekh, Swati ORCID: 0000-0002-4826-1651, Schneider, Farina, Kirchner, Marcel, Kondylis, Vangelis ORCID: 0000-0002-6970-8731, Nikopoulou, Chrysa ORCID: 0000-0001-7982-7737 and Tessarz, Peter (2022). RNA-sequencing of single cholangiocyte-derived organoids reveals high organoid-to organoid variability. Life Sci. Alliance, 5 (12). COLD SPRING HARBOR: LIFE SCIENCE ALLIANCE LLC. ISSN 2575-1077

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Abstract

Over the last decades, organoids have been established from most of the tissue-resident stem and iPS cells. They hold great promise for our understanding of mammalian organ development, but also for the study of disease or even personalised medicine. In recent years, several reports hinted at intraculture organoid variability, but a systematic analysis of such heterogeneity has not been performed before. Here, we used RNA-seq of individual intrahepatic cholangiocyte organoids to address this question. We find that batch-to-batch variation is very low, whereas passage number has a profound impact on gene expression profiles. On the other hand, there is organoid-toorganoid variability within a culture. Using differential gene expression, we did not identify specific pathways that drive this variability, pointing towards possible effects of the microenvironment within the culture condition. Taken together, our study provides a framework for organoid researchers to properly consider experimental design.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Gehling, Kristin UNSPECIFIED orcid.org/0000-0002-7933-3777 UNSPECIFIED Parekh, Swati UNSPECIFIED orcid.org/0000-0002-4826-1651 UNSPECIFIED Schneider, Farina UNSPECIFIED UNSPECIFIED UNSPECIFIED Kirchner, Marcel UNSPECIFIED UNSPECIFIED UNSPECIFIED Kondylis, Vangelis UNSPECIFIED orcid.org/0000-0002-6970-8731 UNSPECIFIED Nikopoulou, Chrysa UNSPECIFIED orcid.org/0000-0001-7982-7737 UNSPECIFIED Tessarz, Peter UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-668886
DOI:	10.26508/lsa.202101340
Journal or Publication Title:	Life Sci. Alliance
Volume:	5
Number:	12
Date:	2022
Publisher:	LIFE SCIENCE ALLIANCE LLC
Place of Publication:	COLD SPRING HARBOR
ISSN:	2575-1077
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELL DIVERSITY; HUMAN LIVER; STEM-CELLS; EXPANSION; CULTURE; PACKAGE; MOUSE Multiple languages Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66888