Universität zu Köln

Maiber, Ludmila, Koprivova, Anna, Bender, Daniel ORCID: 0000-0002-2975-4186, Kopriva, Stanislav ORCID: 0000-0002-7416-6551 and Fischer-Schrader, Katrin (2022). Characterization of the amidoxime reducing components ARC1 and ARC2 from Arabidopsis thaliana. FEBS J., 289 (18). S. 5656 - 5670. HOBOKEN: WILEY. ISSN 1742-4658

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Abstract

Five molybdenum-dependent enzymes are known in eukaryotes. While four of them are under investigation since decades, the most recently discovered, (mitochondrial) amidoxime reducing component ((m)ARC), has only been characterized in mammals and the green algae Chlamydomonas reinhardtii. While mammalian mARCs have been shown to be involved in various signalling pathways, Chlamydomonas ARC was shown to be a nitric oxide (NO)-forming nitrite reductase. Similar to mammals, higher plants possess two ARC proteins. To test whether plant ARCs have a similar function in NO production to the function they have in C. reinhardtii, we analysed the enzymes from the model plant Arabidopsis thaliana. Both ARC1 and ARC2 from Arabidopsis could reduce N-hydroxylated compounds, while nitrite reduction to form NO could only be demonstrated for ARC2. Searching for physiological electron donors, we found that both ARC enzymes accept electrons from NADH via cytochrome b(5) reductase and cytochrome b(5), but only ARC2 is able to accept electrons from nitrate reductase at all. Furthermore, arc-deficient mutant plants were similar to wildtype plants regarding growth and also nitrite-dependent NO-formation. Altogether, our results did not confirm the hypothesis that either ARC1 or ARC2 from Arabidopsis are involved in physiologically relevant nitrite-dependent NO-formation. In contrast, our data suggest that ARC1 and ARC2 have distinct, yet unknown physiological roles in higher plants.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Maiber, Ludmila UNSPECIFIED UNSPECIFIED UNSPECIFIED Koprivova, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Bender, Daniel UNSPECIFIED orcid.org/0000-0002-2975-4186 UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED Fischer-Schrader, Katrin UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-669286
DOI:	10.1111/febs.16450
Journal or Publication Title:	FEBS J.
Volume:	289
Number:	18
Page Range:	S. 5656 - 5670
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1742-4658
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language OXIDE SYNTHASE ACTIVITY; NITRIC-OXIDE; REDUCTASE; IDENTIFICATION; EMISSION; SEQUENCE; PROTEIN; SYSTEM; ENZYME; MUTANT Multiple languages Biochemistry & Molecular Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/66928