Universität zu Köln

Poopalasingam, Nareshkumar, Korenkov, Michael ORCID: 0000-0002-2988-8483, Ashurov, Artem, Strobel, Janina, Fish, Irina, Hellmich, Martin, Gruell, Henning, Lehmann, Clara ORCID: 0000-0002-7042-1578, Heger, Eva and Klein, Florian ORCID: 0000-0003-1376-1792 (2022). Determining the reliability of rapid SARS-CoV-2 antigen detection in fully vaccinated individuals. J. Clin. Virol., 148. AMSTERDAM: ELSEVIER. ISSN 1873-5967

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Abstract

Background: Rapid antigen detection tests (RADT) are commonly used as SARS-CoV-2 diagnostic tests both by medical professionals and laypeople. However, the performance of RADT in vaccinated individuals has not been fully investigated. Objectives: RT-qPCR and rapid antigen detection testing were performed to evaluate the performance of the Standard Q COVID-19 Ag Test in detecting SARS-CoV-2 breakthrough infections in vaccinated individuals.Study design: Two swab specimens, one for RT-qPCR and one for RADT, were collected from vaccinated individuals in an outpatient clinic. For comparison of RADT performance in vaccinated and unvaccinated individuals, a dataset already published by this group was used as reference. Results: During the delta wave, a total of 696 samples were tested with both RT-qPCR and RADT that included 692 (99.4%) samples from vaccinated individuals. Of these, 76 (11.0%) samples were detected SARS-CoV-2 positive by RT-qPCR and 45 (6.5%) samples by the Standard Q COVID-19 Ag test. Stratified by Ct values, sensitivity of the RADT was 100.0%, 94.4% and 81.1% for Ct <= 20 (n=18), Ct <= 25 (n=36) and Ct <= 30 (n=53), respectively. Samples with Ct values >= 30 (n=23) were not detected. Overall RADT specificity was 99.7% and symptom status did not affect RADT performance. Notably, RADT detected 4 out of 4 samples of probable Omicron variant infection based on single nucleotide polymorphism analysis. Conclusion: Our results show that RADT testing remains a valuable tool in detecting breakthrough infections with high viral RNA loads.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Poopalasingam, Nareshkumar UNSPECIFIED UNSPECIFIED UNSPECIFIED Korenkov, Michael UNSPECIFIED orcid.org/0000-0002-2988-8483 UNSPECIFIED Ashurov, Artem UNSPECIFIED UNSPECIFIED UNSPECIFIED Strobel, Janina UNSPECIFIED UNSPECIFIED UNSPECIFIED Fish, Irina UNSPECIFIED UNSPECIFIED UNSPECIFIED Hellmich, Martin UNSPECIFIED UNSPECIFIED UNSPECIFIED Gruell, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Lehmann, Clara UNSPECIFIED orcid.org/0000-0002-7042-1578 UNSPECIFIED Heger, Eva UNSPECIFIED UNSPECIFIED UNSPECIFIED Klein, Florian UNSPECIFIED orcid.org/0000-0003-1376-1792 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-670331
DOI:	10.1016/j.jcv.2022.105119
Journal or Publication Title:	J. Clin. Virol.
Volume:	148
Date:	2022
Publisher:	ELSEVIER
Place of Publication:	AMSTERDAM
ISSN:	1873-5967
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Virology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67033