Universität zu Köln

Pieroth, Stephanie, Heras-Bautista, Carlos O., Hamad, Sarkawt ORCID: 0000-0002-5155-9467, Brockmeier, Konrad, Hescheler, Jurgen, Pfannkuche, Kurt and Schmidt, Annette M. (2023). Poly(acrylamide) Spheroids with Tunable Elasticity for Scalable Cell Culture Applications. Macromol. Chem. Phys., 224 (1). WEINHEIM: WILEY-V C H VERLAG GMBH. ISSN 1521-3935

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Abstract

Many biological tissues resemble hydrogels and display Young's moduli below 50 kPa, corresponding to most cell types for the natural environmental conditions to grow. Contrastingly, conventional cell culture usually involves rigid substrates resulting in stiff priming effects, which are of increasing concern when it comes to scalable culturing of adhesive cells for regenerative purposes. As a solution to this problem, the employment of synthetic poly(acryl amide) (PAAm)-based hydrogel beads with tissue-matched mechanical properties are proposed as soft matrix for culture modes suitable for tidal bioreactor culture. Here, technology is described to generate spherical, mm-scaled PAAm hydrogel beads with adjustable, soft elastic properties that are produced by a continuous microfluidic approach. A simple and robust method is demonstrated to functionalize the spheroids with protein ligands, and the suitability of the matrix for cell cultivation is successfully demonstrated with three different cell types (murine mesenchymal stem cells, renal carcinoma cells, and human induced pluripotent stem cells) in model experiments and in a tidal bioreactor system. This versatile approach will pave the way toward novel cell culture systems based on bioreactors that allow scalable, soft carrier-based expansion of cells on matrices with tissue-matched elasticity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pieroth, Stephanie UNSPECIFIED UNSPECIFIED UNSPECIFIED Heras-Bautista, Carlos O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamad, Sarkawt UNSPECIFIED orcid.org/0000-0002-5155-9467 UNSPECIFIED Brockmeier, Konrad UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Jurgen UNSPECIFIED UNSPECIFIED UNSPECIFIED Pfannkuche, Kurt UNSPECIFIED UNSPECIFIED UNSPECIFIED Schmidt, Annette M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-671132
DOI:	10.1002/macp.202200246
Journal or Publication Title:	Macromol. Chem. Phys.
Volume:	224
Number:	1
Date:	2023
Publisher:	WILEY-V C H VERLAG GMBH
Place of Publication:	WEINHEIM
ISSN:	1521-3935
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MECHANICAL MEMORY; TISSUE; STIFFNESS; MATRIX; BIOREACTOR Multiple languages Polymer Science Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67113