Universität zu Köln

Heinen, Tobias, Xie, Chen, Keshavarz, Maryam, Stappert, Dominik, Kuenzel, Sven and Tautz, Diethard (2022). Evolution of a New Testis-Specific Functional Promoter Within the Highly Conserved Map2k7 Gene of the Mouse. Front. Genet., 12. LAUSANNE: FRONTIERS MEDIA SA. ISSN 1664-8021

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Abstract

Map2k7 (synonym Mkk7) is a conserved regulatory kinase gene and a central component of the JNK signaling cascade with key functions during cellular differentiation. It shows complex transcription patterns, and different transcript isoforms are known in the mouse (Mus musculus). We have previously identified a newly evolved testis-specific transcript for the Map2k7 gene in the subspecies M. m. domesticus. Here, we identify the new promoter that drives this transcript and find that it codes for an open reading frame (ORF) of 50 amino acids. The new promoter was gained in the stem lineage of closely related mouse species but was secondarily lost in the subspecies M. m. musculus and M. m. castaneus. A single mutation can be correlated with its transcriptional activity in M. m. domesticus, and cell culture assays demonstrate the capability of this mutation to drive expression. A mouse knockout line in which the promoter region of the new transcript is deleted reveals a functional contribution of the newly evolved promoter to sperm motility and the spermatid transcriptome. Our data show that a new functional transcript (and possibly protein) can evolve within an otherwise highly conserved gene, supporting the notion of regulatory changes contributing to the emergence of evolutionary novelties.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Heinen, Tobias UNSPECIFIED UNSPECIFIED UNSPECIFIED Xie, Chen UNSPECIFIED UNSPECIFIED UNSPECIFIED Keshavarz, Maryam UNSPECIFIED UNSPECIFIED UNSPECIFIED Stappert, Dominik UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuenzel, Sven UNSPECIFIED UNSPECIFIED UNSPECIFIED Tautz, Diethard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-675207
DOI:	10.3389/fgene.2021.812139
Journal or Publication Title:	Front. Genet.
Volume:	12
Date:	2022
Publisher:	FRONTIERS MEDIA SA
Place of Publication:	LAUSANNE
ISSN:	1664-8021
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PROTEIN-KINASE KINASE; MICE LACKING; SP-10 GENE; PHYSIOLOGICAL ROLES; SIGNAL-TRANSDUCTION; SPERM MATURATION; CORE PROMOTER; TNF-ALPHA; EXPRESSION; MKK7 Multiple languages Genetics & Heredity Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67520