Universität zu Köln

Verma, Ashwani Kumar, Singh, Akanksha, Singh, Rajneesh, Mishra, Prabhatam ORCID: 0000-0002-8168-2503, Narayan, Shiv, Pandey, Vivek ORCID: 0000-0002-3006-7738, Shirke, Pramod Arvind and Roy, Sribash (2022). Population specific methylome remodeling in high and low elevation populations of Indian west Himalayan Arabidopsis thaliana in response to elevated CO2. Environ. Exp. Bot., 203. OXFORD: PERGAMON-ELSEVIER SCIENCE LTD. ISSN 1873-7307

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Abstract

Unravelling the plants responses towards rising atmospheric [CO2] have largely been explored in genetical and morpho-physiological contexts. However, epigenetic factors including DNA methylation which play critical roles in adaptation are largely unexplored. Here we investigated the methylome, transcriptome and morpho-physiological responses of the two Arabidopsis thaliana populations evolved at high (3400 m amsl) and low elevation (700 m amsl) zones to elevated [CO2] (e[CO2]). We show that depending on the origin of the popu-lation, there were local level variations in DNA methylation when exposed to e[CO2], but global methyl cytosine (mC) content remained unchanged. Further, there was loss of methylation and more protein coding genes were differentially methylated than transposable elements and non-coding RNA genes, in both the populations but more so in the low elevation one. The differentially methylated genes of the two populations belonged to distinct functional categories. More genes related to methylation machinery were down-regulated in the high elevation population than the low elevation one. Although there was no correlation between methylation and gene expression at the global scale but a few genes exhibited methylation dependent expression level. Finally the hyper-and hypo-methylated status of a few genes due to e[CO2] treatment were validated. Overall, our data suggested the two populations responded differently towards e[CO2] with respect to methylome remodeling, phenotypic and molecular plasticity. However, methylome remodeling and molecular plasticity were more prominent in the low elevation population. Understanding the evolution of epigenetic response towards e[CO2] may help in future crop improvement strategies.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Verma, Ashwani Kumar UNSPECIFIED UNSPECIFIED UNSPECIFIED Singh, Akanksha UNSPECIFIED UNSPECIFIED UNSPECIFIED Singh, Rajneesh UNSPECIFIED UNSPECIFIED UNSPECIFIED Mishra, Prabhatam UNSPECIFIED orcid.org/0000-0002-8168-2503 UNSPECIFIED Narayan, Shiv UNSPECIFIED UNSPECIFIED UNSPECIFIED Pandey, Vivek UNSPECIFIED orcid.org/0000-0002-3006-7738 UNSPECIFIED Shirke, Pramod Arvind UNSPECIFIED UNSPECIFIED UNSPECIFIED Roy, Sribash UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-675609
DOI:	10.1016/j.envexpbot.2022.105074
Journal or Publication Title:	Environ. Exp. Bot.
Volume:	203
Date:	2022
Publisher:	PERGAMON-ELSEVIER SCIENCE LTD
Place of Publication:	OXFORD
ISSN:	1873-7307
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language DNA METHYLATION; STRESS TOLERANCE; CYTOSINE METHYLATION; GENE-EXPRESSION; ATMOSPHERIC CO2; PLANT-GROWTH; CROP YIELD; GENOME; DEMETHYLATION; PROTEIN Multiple languages Plant Sciences; Environmental Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67560