Universität zu Köln

Raspa, Marcello, Paoletti, Renata, Peltier, Manon, Majjouti, Mohamed, Protti, Michele ORCID: 0000-0001-9310-4957, Mercolini, Laura, Mahabir, Esther and Scavizzi, Ferdinando (2022). Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice. Animals, 12 (11). BASEL: MDPI. ISSN 2076-2615

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Abstract

Simple Summary Investigations concerning the impact of D-Aspartate on fertility suggest that it has a positive influence on the in vitro fertilization rate in young C57BL/6N mice. Here, we demonstrated that adult C57BL/6N mice that received an oral treatment of D-Aspartate also have a higher fertilizing capability and the quality of their spermatozoa increased after only two weeks of treatment. Hence, this study gives us new insights on the role of D-Aspartate in the regulation of the reproductive activity in both young and adult mice. D-Aspartate (D-Asp) treatment improved the fertility of young male C57BL/6N mice in vivo revealing a direct role on capacitation, acrosome reaction, and fertility in vitro in young males only. We investigated whether the positive effect of D-Asp on fertility could be extended to adult males and evaluated the efficacy of a 2- or 4-week-treatment in vivo. Therefore, 20 mM sodium D-Asp was supplied in drinking water to males of different ages so that they were 9 or 16 weeks old at the end of the experiments. After sperm freezing, the in vitro fertilization (IVF) rate, the birth rate, hormone levels (luteinizing hormone (LH), epitestosterone, and testosterone), the sperm quality (morphology, abnormalities, motility, and velocity), the capacitation rate, and the acrosome reaction were investigated. Oral D-Asp treatment improves the fertilizing capability in mice regardless of the age of the animals. Importantly, a short D-Asp treatment of 2 weeks in young males elevates sperm parameters to the levels of untreated adult animals. In vivo, D-Asp treatment highly improves sperm quality but not sperm concentration. Therefore, D-Asp plays a beneficial role in mouse male fertility and may be highly relevant for cryorepositories to improve mouse sperm biobanking.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Raspa, Marcello UNSPECIFIED UNSPECIFIED UNSPECIFIED Paoletti, Renata UNSPECIFIED UNSPECIFIED UNSPECIFIED Peltier, Manon UNSPECIFIED UNSPECIFIED UNSPECIFIED Majjouti, Mohamed UNSPECIFIED UNSPECIFIED UNSPECIFIED Protti, Michele UNSPECIFIED orcid.org/0000-0001-9310-4957 UNSPECIFIED Mercolini, Laura UNSPECIFIED UNSPECIFIED UNSPECIFIED Mahabir, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Scavizzi, Ferdinando UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-677407
DOI:	10.3390/ani12111350
Journal or Publication Title:	Animals
Volume:	12
Number:	11
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2076-2615
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language IN-VITRO FERTILIZATION; PLASMA TESTOSTERONE LEVELS; MOUSE SPERM; TYROSINE PHOSPHORYLATION; ACROSOME REACTION; HUMAN SPERMATOZOA; ZONA-PELLUCIDA; CAPACITATION; QUALITY; ACID Multiple languages Agriculture, Dairy & Animal Science; Veterinary Sciences; Zoology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/67740