Universität zu Köln

Szeles, Eszter ORCID: 0000-0003-0800-9571, Nagy, Krisztina, Abraham, Agnes, Kovacs, Sandor, Podmaniczki, Anna, Nagy, Valeria, Kovacs, Laszlo, Galajda, Peter ORCID: 0000-0003-3308-088X and Toth, Szilvia Z. ORCID: 0000-0003-3419-829X (2022). Microfluidic Platforms Designed for Morphological and Photosynthetic Investigations of Chlamydomonas reinhardtii on a Single-Cell Level. Cells, 11 (2). BASEL: MDPI. ISSN 2073-4409

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Abstract

Chlamydomonas reinhardtii is a model organism of increasing biotechnological importance, yet, the evaluation of its life cycle processes and photosynthesis on a single-cell level is largely unresolved. To facilitate the study of the relationship between morphology and photochemistry, we established microfluidics in combination with chlorophyll a fluorescence induction measurements. We developed two types of microfluidic platforms for single-cell investigations: (i) The traps of the Tulip device are suitable for capturing and immobilizing single cells, enabling the assessment of their photosynthesis for several hours without binding to a solid support surface. Using this Tulip platform, we performed high-quality non-photochemical quenching measurements and confirmed our earlier results on bulk cultures that non-photochemical quenching is higher in ascorbate-deficient mutants (Crvtc2-1) than in the wild-type. (ii) The traps of the Pot device were designed for capturing single cells and allowing the growth of the daughter cells within the traps. Using our most performant Pot device, we could demonstrate that the F-V/F-M parameter, an indicator of photosynthetic efficiency, varies considerably during the cell cycle. Our microfluidic devices, therefore, represent versatile platforms for the simultaneous morphological and photosynthetic investigations of C. reinhardtii on a single-cell level.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Szeles, Eszter UNSPECIFIED orcid.org/0000-0003-0800-9571 UNSPECIFIED Nagy, Krisztina UNSPECIFIED UNSPECIFIED UNSPECIFIED Abraham, Agnes UNSPECIFIED UNSPECIFIED UNSPECIFIED Kovacs, Sandor UNSPECIFIED UNSPECIFIED UNSPECIFIED Podmaniczki, Anna UNSPECIFIED UNSPECIFIED UNSPECIFIED Nagy, Valeria UNSPECIFIED UNSPECIFIED UNSPECIFIED Kovacs, Laszlo UNSPECIFIED UNSPECIFIED UNSPECIFIED Galajda, Peter UNSPECIFIED orcid.org/0000-0003-3308-088X UNSPECIFIED Toth, Szilvia Z. UNSPECIFIED orcid.org/0000-0003-3419-829X UNSPECIFIED
URN:	urn:nbn:de:hbz:38-680931
DOI:	10.3390/cells11020285
Journal or Publication Title:	Cells
Volume:	11
Number:	2
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2073-4409
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SOFT LITHOGRAPHY; GROWTH-RATE; BIOTECHNOLOGY; BIOSENSOR; CULTURE; SYSTEMS; DEVICE; SIZER; CHIP Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68093