Universität zu Köln

Kraemer, Jonas, Lueddecke, Tim, Marner, Michael ORCID: 0000-0002-1024-1567, Maiworm, Elena, Eichberg, Johanna, Hardes, Kornelia, Schaeberle, Till F., Vilcinskas, Andreas ORCID: 0000-0001-8276-4968 and Predel, Reinhard (2022). Antimicrobial, Insecticidal and Cytotoxic Activity of Linear Venom Peptides from the Pseudoscorpion Chelifer cancroides. Toxins, 14 (1). BASEL: MDPI. ISSN 2072-6651

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Abstract

Linear cationic venom peptides are antimicrobial peptides (AMPs) that exert their effects by damaging cell membranes. These peptides can be highly specific, and for some, a significant therapeutic value was proposed, in particular for treatment of bacterial infections. A prolific source of novel AMPs are arthropod venoms, especially those of hitherto neglected groups such as pseudoscorpions. In this study, we describe for the first time pharmacological effects of AMPs discovered in pseudoscorpion venom. We examined the antimicrobial, cytotoxic, and insecticidal activity of full-length Checacin1, a major component of the Chelifer cancroides venom, and three truncated forms of this peptide. The antimicrobial tests revealed a potent inhibitory activity of Checacin1 against several bacteria and fungi, including methicillin resistant Staphylococcus aureus (MRSA) and even Gram-negative pathogens. All peptides reduced survival rates of aphids, with Checacin1 and the C-terminally truncated Checacin1(1-21) exhibiting effects comparable to Spinosad, a commercially used pesticide. Cytotoxic effects on mammalian cells were observed mainly for the full-length Checacin1. All tested peptides might be potential candidates for developing lead structures for aphid pest treatment. However, as these peptides were not yet tested on other insects, aphid specificity has not been proven. The N- and C-terminal fragments of Checacin1 are less potent against aphids but exhibit no cytotoxicity on mammalian cells at the tested concentration of 100 mu M.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kraemer, Jonas UNSPECIFIED UNSPECIFIED UNSPECIFIED Lueddecke, Tim UNSPECIFIED UNSPECIFIED UNSPECIFIED Marner, Michael UNSPECIFIED orcid.org/0000-0002-1024-1567 UNSPECIFIED Maiworm, Elena UNSPECIFIED UNSPECIFIED UNSPECIFIED Eichberg, Johanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Hardes, Kornelia UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaeberle, Till F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Vilcinskas, Andreas UNSPECIFIED orcid.org/0000-0001-8276-4968 UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-681738
DOI:	10.3390/toxins14010058
Journal or Publication Title:	Toxins
Volume:	14
Number:	1
Date:	2022
Publisher:	MDPI
Place of Publication:	BASEL
ISSN:	2072-6651
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELLS Multiple languages Food Science & Technology; Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68173