Kretschmer, Alina Chloe, Junker, Lena, Dewald, Felix, Linne, Viktoria, Hennen, Lea, Horemheb-Rubio, Gibran, Kaiser, Rolf ORCID: 0000-0002-2640-2548, Steger, Gertrud, Joachim, Alexander, Schoenenkorb, Jana, Cosgun, Zuelfue Cem, Muehlhans, Neslihan, Heger, Eva, Knops, Elena, Leisse, Charlotte, Kessel, Barbora, Heinsohn, Torben, Rodiah, Isti, Lange, Berit, Ritter, Anne Lena, Fries, Mira, Kossow, Annelene, Niessen, Johannes, Doetsch, Joerg, Klein, Florian ORCID: 0000-0003-1376-1792, Rybniker, Jan, Faetkenheuer, Gerd and Suarez, Isabelle . Implementing the Lolli-Method and pooled RT-qPCR testing for SARS-CoV-2 surveillance in schools: a pilot project. Infection. HEIDELBERG: SPRINGER HEIDELBERG. ISSN 1439-0973

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Abstract

Purpose School closures have been used as part of lockdown strategies to contain the spread of SARS-CoV-2, adversely affecting children's health and education. To ensure the accessibility of educational institutions without exposing society to the risk of increased transmissions, it is essential to establish SARS-CoV-2 testing strategies that are child-friendly, scalable and implementable in a daily school routine. Self-sampling using non-invasive saliva swabs combined with pooled RT-qPCR testing (Lolli-Method) has been proven to be a sensitive method for the detection of SARS-CoV-2. Methods We conducted a pilot project in Cologne, Germany, designed to determine the feasibility of a large-scale rollout of the Lolli-Method for testing without any additional on-site medical staff in schools. Over a period of three weeks, students from 22 schools were sampled using the Lolli-Method. At the end of the project, teachers were asked to evaluate the overall acceptance of the project. Results We analyzed a total of 757 pooled RT-qPCRs obtained from 8,287 individual swabs and detected 7 SARS-CoV-2 infected individuals. The Lolli-Method was shown to be a feasible and accepted testing strategy whose application is only slightly disruptive to the daily school routine. Conclusion Our observations suggest that the Lolli-Method in combination with pooled RT-qPCR can be implemented for SARS-CoV-2 surveillance in daily school routine, applicable on a large scale.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Kretschmer, Alina ChloeUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Junker, LenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dewald, FelixUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Linne, ViktoriaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hennen, LeaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Horemheb-Rubio, GibranUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kaiser, RolfUNSPECIFIEDorcid.org/0000-0002-2640-2548UNSPECIFIED
Steger, GertrudUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Joachim, AlexanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schoenenkorb, JanaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Cosgun, Zuelfue CemUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Muehlhans, NeslihanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heger, EvaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Knops, ElenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Leisse, CharlotteUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kessel, BarboraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Heinsohn, TorbenUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rodiah, IstiUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Lange, BeritUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ritter, Anne LenaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fries, MiraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kossow, AnneleneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Niessen, JohannesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doetsch, JoergUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Klein, FlorianUNSPECIFIEDorcid.org/0000-0003-1376-1792UNSPECIFIED
Rybniker, JanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Faetkenheuer, GerdUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Suarez, IsabelleUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-682478
DOI: 10.1007/s15010-022-01865-0
Journal or Publication Title: Infection
Publisher: SPRINGER HEIDELBERG
Place of Publication: HEIDELBERG
ISSN: 1439-0973
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
COVID-19Multiple languages
Infectious DiseasesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/68247

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