Universität zu Köln

Sattler, Janko ORCID: 0000-0003-1415-4169, Tsvetkov, Tsvetan, Stelzer, Yvonne, Schaefer, Sina, Sommer, Julian, Noster, Janina ORCID: 0000-0002-8802-6605, Goettig, Stephan and Hamprecht, Axel (2022). Emergence of Tn1999.7, a New Transposon in bla(OXA-48)-Harboring Plasmids Associated with Increased Plasmid Stability 1999.7. Antimicrob. Agents Chemother., 66 (11). WASHINGTON: AMER SOC MICROBIOLOGY. ISSN 1098-6596

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Abstract

OXA-48 is the most common carbapenemase in Enterobacterales in Germany and many other European countries. Depending on the genomic location of bla(OXA-48), OXA-48-producing isolates vary in phenotype and intra- and interspecies transferability of bla(OXA-48). In most bacterial isolates, bla(OXA-48) is located on one of seven variants of Tn1999 (Tn1999.1 to Tn1999.6 and invTn1999.2). Here, a novel Tn1999 variant, Tn1999.7, is described, which was identified in 11 clinical isolates from 2016 to 2020. Tn1999.7 differs from Tn1999.1 by the insertion of the 8,349-bp Tn3 family transposon Tn7442 between the lysR gene and bla(OXA-48) open reading frame. Tn7442 carries genes coding for a restriction endonuclease and a DNA methyltransferase as cargo, forming a type III restriction modification system. Tn1999.7 was carried on an similar to 71-kb IncL plasmid in 9/11 isolates. In one isolate, Tn1999.7 was situated on an similar to 76-kb plasmid, harboring an additional insertion sequence in the plasmid backbone. In one isolate, the plasmid size is only similar to 63 kb due to a deletion adjacent to Tn7442 that extends into the plasmid backbone. Mean conjugation rates of the Tn1999.7-harboring plasmids in J53 ranged from 4.47 x 10(-5) to 2.03 x 10(-2), similar to conjugation rates of other pOXA-48-type IncL plasmids. The stability of plasmids with Tn1999.7 was significantly higher than that of a Tn1999.2-harboring plasmid in vitro. This increase in stability could be related to the insertion of a restriction-modification system, which can promote postsegregational killing. The increased plasmid stability associated with Tn1999.7 could contribute to the further spread of OXA-48.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Sattler, Janko UNSPECIFIED orcid.org/0000-0003-1415-4169 UNSPECIFIED Tsvetkov, Tsvetan UNSPECIFIED UNSPECIFIED UNSPECIFIED Stelzer, Yvonne UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaefer, Sina UNSPECIFIED UNSPECIFIED UNSPECIFIED Sommer, Julian UNSPECIFIED UNSPECIFIED UNSPECIFIED Noster, Janina UNSPECIFIED orcid.org/0000-0002-8802-6605 UNSPECIFIED Goettig, Stephan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hamprecht, Axel UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-682668
DOI:	10.1128/aac.00787-22
Journal or Publication Title:	Antimicrob. Agents Chemother.
Volume:	66
Number:	11
Date:	2022
Publisher:	AMER SOC MICROBIOLOGY
Place of Publication:	WASHINGTON
ISSN:	1098-6596
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ESCHERICHIA-COLI; SPREAD; RESTRICTION; OXA-48; PATHOGENICITY; INSERTION Multiple languages Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68266