Universität zu Köln

Pavlu, Jaroslav ORCID: 0000-0002-7855-8907, Kerchev, Pavel, Cerny, Martin ORCID: 0000-0002-0651-4219, Novak, Jan ORCID: 0000-0001-6248-5355, Berka, Miroslav ORCID: 0000-0002-7682-2778, Jobe, Timothy O., Lopez Ramos, Jose Maria, Saiz-Fernandez, Inigo, Rashotte, Aaron Michael, Kopriva, Stanislav ORCID: 0000-0002-7416-6551 and Brzobohaty, Bretislav ORCID: 0000-0002-1552-1314 (2022). Cytokinin modulates the metabolic network of sulfur and glutathione. J. Exp. Bot., 73 (22). S. 7417 - 7434. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2431

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Abstract

The phytohormone cytokinin is implicated in a range of growth, developmental, and defense processes. A growing body of evidence supports a crosstalk between cytokinin and nutrient signaling pathways, such as nitrate availability. Cytokinin signaling regulates sulfur-responsive gene expression, but the underlying molecular mechanisms and their impact on sulfur-containing metabolites have not been systematically explored. Using a combination of genetic and pharmacological tools, we investigated the interplay between cytokinin signaling and sulfur homeostasis. Exogenous cytokinin triggered sulfur starvation-like gene expression accompanied by a decrease in sulfate and glutathione content. This process was uncoupled from the activity of the major transcriptional regulator of sulfate starvation signaling SULFUR LIMITATION 1 and an important glutathione-degrading enzyme, gamma-glutamyl cyclotransferase 2;1, expression of which was robustly up-regulated by cytokinin. Conversely, glutathione accumulation was observed in mutants lacking the cytokinin receptor ARABIDOPSIS HISTIDINE KINASE 3 and in cytokinin-deficient plants. Cytokinin-deficient plants displayed improved root growth upon exposure to glutathione-depleting chemicals which was attributed to a higher capacity to maintain glutathione levels. These results shed new light on the interplay between cytokinin signaling and sulfur homeostasis. They position cytokinin as an important modulator of sulfur uptake, assimilation, and remobilization in plant defense against xenobiotics and root growth. Cytokinins induce transcriptional changes similar to sulfate starvation response and modulate the contents of sulfur-containing metabolites.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Pavlu, Jaroslav UNSPECIFIED orcid.org/0000-0002-7855-8907 UNSPECIFIED Kerchev, Pavel UNSPECIFIED UNSPECIFIED UNSPECIFIED Cerny, Martin UNSPECIFIED orcid.org/0000-0002-0651-4219 UNSPECIFIED Novak, Jan UNSPECIFIED orcid.org/0000-0001-6248-5355 UNSPECIFIED Berka, Miroslav UNSPECIFIED orcid.org/0000-0002-7682-2778 UNSPECIFIED Jobe, Timothy O. UNSPECIFIED UNSPECIFIED UNSPECIFIED Lopez Ramos, Jose Maria UNSPECIFIED UNSPECIFIED UNSPECIFIED Saiz-Fernandez, Inigo UNSPECIFIED UNSPECIFIED UNSPECIFIED Rashotte, Aaron Michael UNSPECIFIED UNSPECIFIED UNSPECIFIED Kopriva, Stanislav UNSPECIFIED orcid.org/0000-0002-7416-6551 UNSPECIFIED Brzobohaty, Bretislav UNSPECIFIED orcid.org/0000-0002-1552-1314 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-684049
DOI:	10.1093/jxb/erac391
Journal or Publication Title:	J. Exp. Bot.
Volume:	73
Number:	22
Page Range:	S. 7417 - 7434
Date:	2022
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2431
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language PHOSPHATE STARVATION RESPONSES; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; SULFATE ASSIMILATION; BIOSYNTHESIS; ROOT; REVEALS; AUXIN; REGULATOR; MUTANTS Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68404