Universität zu Köln

Schenk, Martijn F., Zwart, Mark P., Hwang, Sungmin, Ruelens, Philip, Severing, Edouard, Krug, Joachim and de Visser, J. Arjan G. M. (2022). Population size mediates the contribution of high-rate and large-benefit mutations to parallel evolution. Nat. Ecol. Evol., 6 (4). S. 439 - 457. BERLIN: NATURE PORTFOLIO. ISSN 2397-334X

Full text not available from this repository.

Abstract

Mutations with large fitness benefits and mutations occurring at high rates may both cause parallel evolution, but their contribution is predicted to depend on population size. Moreover, high-rate and large-benefit mutations may have different long-term adaptive consequences. We show that small and 100-fold larger bacterial populations evolve resistance to a beta-lactam antibiotic by using similar numbers, but different types of mutations. Small populations frequently substitute similar high-rate structural variants and loss-of-function point mutations, including the deletion of a low-activity beta-lactamase, and evolve modest resistance levels. Large populations more often use low-rate, large-benefit point mutations affecting the same targets, including mutations activating the beta-lactamase and other gain-of-function mutations, leading to much higher resistance levels. Our results demonstrate the separation by clonal interference of mutation classes with divergent adaptive consequences, causing a shift from high-rate to large-benefit mutations with increases in population size. Population size influences mutation supply but may also influence what types of mutations eventually become fixed. Here, Schenk et al. show that small and large experimentally evolving bacteria populations predictably fix different types of mutations, with greater antibiotic resistance emerging in large populations.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Schenk, Martijn F. UNSPECIFIED UNSPECIFIED UNSPECIFIED Zwart, Mark P. UNSPECIFIED UNSPECIFIED UNSPECIFIED Hwang, Sungmin UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruelens, Philip UNSPECIFIED UNSPECIFIED UNSPECIFIED Severing, Edouard UNSPECIFIED UNSPECIFIED UNSPECIFIED Krug, Joachim UNSPECIFIED UNSPECIFIED UNSPECIFIED de Visser, J. Arjan G. M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-684188
DOI:	10.1038/s41559-022-01669-3
Journal or Publication Title:	Nat. Ecol. Evol.
Volume:	6
Number:	4
Page Range:	S. 439 - 457
Date:	2022
Publisher:	NATURE PORTFOLIO
Place of Publication:	BERLIN
ISSN:	2397-334X
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MOLECULAR EVOLUTION; DIMINISHING RETURNS; GENE AMPLIFICATION; NATURAL EVOLUTION; ESCHERICHIA-COLI; ADAPTATION; DYNAMICS; PREDICTABILITY; RESISTANCE; SELECTION Multiple languages Ecology; Evolutionary Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68418