Universität zu Köln

Ortiz, Diana, Chen, Jian ORCID: 0000-0001-8670-6984, Outram, Megan A., Saur, Isabel M. L., Upadhyaya, Narayana M., Mago, Rohit ORCID: 0000-0001-5286-073X, Ericsson, Daniel J., Cesari, Stella, Chen, Chunhong ORCID: 0000-0001-7813-2415, Williams, Simon J. and Dodds, Peter N. (2022). The stem rust effector protein AvrSr50 escapes Sr50 recognition by a substitution in a single surface-exposed residue. New Phytol., 234 (2). S. 592 - 607. HOBOKEN: WILEY. ISSN 1469-8137

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Abstract

Pathogen effectors are crucial players during plant colonisation and infection. Plant resistance mostly relies on effector recognition to activate defence responses. Understanding how effector proteins escape from plant surveillance is important for plant breeding and resistance deployment. Here we examined the role of genetic diversity of the stem rust (Puccinia graminis f. sp. tritici (Pgt)) AvrSr50 gene in determining recognition by the corresponding wheat Sr50 resistance gene. We solved the crystal structure of a natural variant of AvrSr50 and used site-directed mutagenesis and transient expression assays to dissect the molecular mechanisms explaining gain of virulence. We report that AvrSr50 can escape recognition by Sr50 through different mechanisms including DNA insertion, stop codon loss or by amino-acid variation involving a single substitution of the AvrSr50 surface-exposed residue Q121. We also report structural homology of AvrSr50 to cupin superfamily members and carbohydrate-binding modules indicating a potential role in binding sugar moieties. This study identifies key polymorphic sites present in AvrSr50 alleles from natural stem rust populations that play important roles to escape from Sr50 recognition. This constitutes an important step to better understand Pgt effector evolution and to monitor AvrSr50 variants in natural rust populations.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Ortiz, Diana UNSPECIFIED UNSPECIFIED UNSPECIFIED Chen, Jian UNSPECIFIED orcid.org/0000-0001-8670-6984 UNSPECIFIED Outram, Megan A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Saur, Isabel M. L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Upadhyaya, Narayana M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Mago, Rohit UNSPECIFIED orcid.org/0000-0001-5286-073X UNSPECIFIED Ericsson, Daniel J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Cesari, Stella UNSPECIFIED UNSPECIFIED UNSPECIFIED Chen, Chunhong UNSPECIFIED orcid.org/0000-0001-7813-2415 UNSPECIFIED Williams, Simon J. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dodds, Peter N. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-689911
DOI:	10.1111/nph.18011
Journal or Publication Title:	New Phytol.
Volume:	234
Number:	2
Page Range:	S. 592 - 607
Date:	2022
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1469-8137
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language MACROMOLECULAR CRYSTALLOGRAPHY COMMUNITIES; FLAX-RUST; RESISTANCE GENES; STRUCTURE REFINEMENT; CONFERS RESISTANCE; WHEAT GENE; PLANT; ASSOCIATION; DOMAIN; IDENTIFICATION Multiple languages Plant Sciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/68991