Soultoukis, Georgios (2016). Exome-matching of dietary amino acids in flies and mice. PhD thesis, Universität zu Köln.
|
PDF
George_Soultoukis_PhD_Thesis_10012017.pdf Download (32MB) |
Abstract
Dietary restriction (DR) is a moderate reduction in nutrient intake that improves health and prolongs longevity across most species tested. The restriction of specific nutrients, rather than overall food intake, is particularly important in mediating the beneficial effects of DR. In flies dietary protein mediates DR-induced lifespan-extension, and imbalance of single amino acids (AAs) dictates longevity and fecundity. In a conserved fashion in mice and rats, restriction of protein or of single essential AAs (EAAs) also extends lifespan. However, the definition of what constitutes a limitation or excess in the intake of an AA can be dubious as many past attempts to determine a reliable measure for dietary AA requirements have had limited success. A possible general predictor of the AA requirement of an organism could be its genome, potentially allowing a rational design for a balanced dietary AA ratio. Here it is reported that the exome composition of an organism may provide a suitable template for its AA requirements. Matching the dietary AA supply of flies or mice to their exome promotes anabolic traits including growth and fecundity compared to amino acid profiles found in commonly used protein sources including yeast and casein. In flies, the presence of exome-defined limiting amino acids lowers the amount of bioavailable protein in the diet. However this response also depended on the identity of the limiting amino acid. Therefore the amino acid profile of a food source is an important factor when considering the usage of dietary nitrogen, anabolic traits, and lifespan.
Item Type: | Thesis (PhD thesis) | ||||||||
Translated abstract: |
|
||||||||
Creators: |
|
||||||||
Corporate Creators: | Max Planck Institute for Biology of Ageing, Cologne, Graduate School for Biological Sciences, University of Cologne | ||||||||
URN: | urn:nbn:de:hbz:38-71307 | ||||||||
Date: | 20 December 2016 | ||||||||
Language: | English | ||||||||
Faculty: | Faculty of Mathematics and Natural Sciences | ||||||||
Divisions: | Außeruniversitäre Forschungseinrichtungen > MPI for Biology of Ageing | ||||||||
Subjects: | Natural sciences and mathematics Life sciences Medical sciences Medicine |
||||||||
Uncontrolled Keywords: |
|
||||||||
Date of oral exam: | 3 June 2016 | ||||||||
Referee: |
|
||||||||
Funders: | Max Planck Gesellschaft, European Research Counsil | ||||||||
Refereed: | Yes | ||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/7130 |
Downloads
Downloads per month over past year
Export
Actions (login required)
View Item |