Universität zu Köln

Gehlsen, Uta ORCID: 0000-0001-9057-9199, Maass, Martina, Stary, Daniela, Wagener-Ryczek, Svenja ORCID: 0000-0002-2870-5606, Musial, Gwen ORCID: 0000-0003-4143-2902, Pasparakis, Manolis ORCID: 0000-0002-9870-0966, de Paiva, Cintia S., Stern, Michael E. ORCID: 0000-0001-7466-1184 and Steven, Philipp ORCID: 0000-0001-6892-3619 (2025). Dessicating stress triggers and exacerbates experimental ocular Graft-versus-host-disease. The Ocular Surface, 37. pp. 236-246. Elsevier. ISSN 15420124

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Abstract

Introduction: Chronic ocular graft-versus-host disease (oGVHD) is one of the most common complications after allogeneic hematopoietic stem cell transplantation (aHSCT). Recent studies indicate that desiccating stress by air-conditioning in transplantation wards increases the incidence of oGVHD. To test the hypothesis that experimental desiccating stress is a risk factor for oGVHD a mouse model of oGVHD was subjected to experimental desiccating stress. Materials/methods: A previously established chemo-induced minor-mismatch mouse model of oGVHD was used. One group was challenged with desiccating stress for 18 days and compared to non-desiccated GVHD animals. Clinical phenotyping was performed weekly and ocular tissue and regional lymph nodes were collected on days 7 and 28 for flow-cytometry, tear film cytokine analysis, histology for corneal lymphatics and dendritic cell counts, and corneal gene expression. Results: Desiccating stress leads to significant earlier and more severe systemic and oGVHD accompanied by higher numbers of activated corneal dendritic cells, higher expression of TNF in tear film and earlier corneal lymphangiogenesis. Gene expression analysis suggests that systemic GVHD severity may influence oGVHD. Different inflammatory pathways are upregulated at d28 following desiccating stress in contrast to non-desiccated GVHD. Conclusions: The data presented strengthens the hypothesis, that desiccating stress during aHSCT is a risk factor for oGVHD. Together with already published clinical data, there is increasing evidence that implicates protecting patients from desiccation during the engraftment of allogeneic hematopoietic stem cells. Furthermore, specific prophylactic therapies should be developed and tested to reduce the incidence and severity of oGVHD.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Gehlsen, Uta UNSPECIFIED https://orcid.org/0000-0001-9057-9199 UNSPECIFIED Maass, Martina UNSPECIFIED UNSPECIFIED UNSPECIFIED Stary, Daniela UNSPECIFIED UNSPECIFIED UNSPECIFIED Wagener-Ryczek, Svenja UNSPECIFIED https://orcid.org/0000-0002-2870-5606 UNSPECIFIED Musial, Gwen UNSPECIFIED https://orcid.org/0000-0003-4143-2902 UNSPECIFIED Pasparakis, Manolis UNSPECIFIED https://orcid.org/0000-0002-9870-0966 UNSPECIFIED de Paiva, Cintia S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Stern, Michael E. UNSPECIFIED https://orcid.org/0000-0001-7466-1184 UNSPECIFIED Steven, Philipp UNSPECIFIED https://orcid.org/0000-0001-6892-3619 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-803551
Identification Number:	10.1016/j.jtos.2025.04.008
Journal or Publication Title:	The Ocular Surface
Volume:	37
Page Range:	pp. 236-246
Number of Pages:	11
Date:	July 2025
Publisher:	Elsevier
ISSN:	15420124
Language:	English
Faculty:	Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases Faculty of Medicine > Innere Medizin > Klinik I für Innere Medizin - Hämatologie und Onkologie Faculty of Medicine > Pathologie und Neuropathologie > Institut für Pathologie Zentrum für Molekulare Medizin
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language Ocular graft-versus-host disease ; Desiccating stress ; Allogeneic hematopoietic stem cell ; transplantation ; Risk factor English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/80355