Universität zu Köln

Habbig, Sandra ORCID: 0000-0002-6511-5023, Debiec, Hanna, Chedik, Malha, Stippel, Dirk L., Erger, Florian ORCID: 0000-0002-2768-1702, Lourenço, Alexia, Liebau, Max C. ORCID: 0000-0003-0494-9080 and Ronco, Pierre ORCID: 0000-0002-0280-6900 (2025). Anti-nephrin antibodies guide living donor kidney transplantation in a pediatric patient with primary focal segmental glomerular sclerosis. Kidney International, 108 (2). pp. 321-327. Elsevier. ISSN 00852538

PDF PIIS008525382500393X.pdf Bereitstellung unter der CC-Lizenz: Creative Commons Attribution. Download (1MB)

Abstract

Introduction: Disease recurrence after kidney transplantation (KTx) remains a major challenge in patients with primary focal segmental glomerulosclerosis (pFSGS). Antibodies targeting the slit diaphragm protein nephrin have been identified in patients with early disease recurrence. Here, we describe monitoring and effective pre-transplant elimination of anti-nephrin antibodies in an adolescent with pFSGS prior to living-donor KTx. Methods: Anti-nephrin antibodies were assessed in pre- and post-transplant serum samples by ELISA, Western blot and immunoprecipitation using three different nephrin proteins. Results: Pre-transplant treatment including rituximab and repetitive therapeutic plasma exchanges resulted in effective and sustainable reduction of anti-nephrin antibodies. Allograft function has remained excellent without albuminuria over a follow-up of more than one year. Further analysis showed that the antibodies were cross-reactive with NEPH3 (filtrin), another key slit diaphragm protein. Conclusions: Monitoring and pre-transplant elimination of anti–slit diaphragm antibodies may become a standard, personalized approach in patients with pFSGS requiring KTx.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Habbig, Sandra UNSPECIFIED https://orcid.org/0000-0002-6511-5023 UNSPECIFIED Debiec, Hanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Chedik, Malha UNSPECIFIED UNSPECIFIED UNSPECIFIED Stippel, Dirk L. UNSPECIFIED UNSPECIFIED UNSPECIFIED Erger, Florian UNSPECIFIED https://orcid.org/0000-0002-2768-1702 UNSPECIFIED Lourenço, Alexia UNSPECIFIED UNSPECIFIED UNSPECIFIED Liebau, Max C. UNSPECIFIED https://orcid.org/0000-0003-0494-9080 UNSPECIFIED Ronco, Pierre UNSPECIFIED https://orcid.org/0000-0002-0280-6900 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-803587
Identification Number:	10.1016/j.kint.2025.04.015
Journal or Publication Title:	Kidney International
Volume:	108
Number:	2
Page Range:	pp. 321-327
Number of Pages:	7
Date:	August 2025
Publisher:	Elsevier
ISSN:	00852538
Language:	English
Faculty:	Faculty of Medicine
Divisions:	Faculty of Medicine > Chirurgie > Klinik und Poliklinik für Allgemein-, Viszeral-, Thorax- und Transplantationschirurgie Faculty of Medicine > Frauenheilkunde > Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe Faculty of Medicine > Humangenetik > Institut für Humangenetik Faculty of Medicine > Kinder- und Jugendmedizin > Klinik und Poliklinik für Kinder- und Jugendmedizin Faculty of Medicine > Weitere > Zentrum für seltene Erkrankungen Zentrum für Molekulare Medizin
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language anti-NEPH3 antibodies ; anti-nephrin antibodies ; primary FSGS ; slit diaphragm English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/80358