Georgiev, Tihomir
(2025).
Prediction of fast subcellular calcium signals based on label-free second harmonic generation microscopy measurements.
Computers in Biology and Medicine, 198.
pp. 1-13.
Elsevier.
ISSN 0010-4825
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Abstract
[Artikel-Nr.: 111138] Calcium (Ca2+) sparks were predicted based on the label-free second harmonic generation (SHG) signal of myosin filaments with pix2pix, a conditional adversarial network. Predicted Ca2+ sparks and ground truth Ca2+ sparks show similar properties. Predicted Ca2+ spark frequencies and a predominant location at the periphery of skeletal muscle fibers were in good agreement with ground truth data. High frequencies of predicted Ca2+ sparks could be observed at y-shaped structures of myosin filaments. Important image pre-processing was applied in order to enhance the signal of Ca2+ sparks used for training. Only open-source software was applied. I provide the image pre-processing algorithm and several algorithms to evaluate the quality of predicted Ca2+ sparks. This technique can be extended to other label-free microscopy techniques and other fluorescent indicators developed during the last years. It remains a difficult task to predict the exact location of Ca2+ sparks.
| Item Type: | Article |
| Creators: | Creators Email ORCID ORCID Put Code Georgiev, Tihomir UNSPECIFIED UNSPECIFIED UNSPECIFIED |
| URN: | urn:nbn:de:hbz:38-810844 |
| Identification Number: | 10.1016/j.compbiomed.2025.111138 |
| Journal or Publication Title: | Computers in Biology and Medicine |
| Volume: | 198 |
| Page Range: | pp. 1-13 |
| Number of Pages: | 13 |
| Date: | November 2025 |
| Publisher: | Elsevier |
| ISSN: | 0010-4825 |
| Language: | English |
| Faculty: | Faculty of Medicine |
| Divisions: | Faculty of Medicine > Augenheilkunde > Klinik und Poliklinik für Allgemeine Augenheilkunde |
| Subjects: | Medical sciences Medicine |
| ['eprint_fieldname_oa_funders' not defined]: | Publikationsfonds UzK |
| Refereed: | Yes |
| URI: | http://kups.ub.uni-koeln.de/id/eprint/81084 |
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