Universität zu Köln

Beielstein, Anna C. ORCID: 0000-0001-7710-1377, Blakemore, Stuart ORCID: 0000-0003-2892-1162 and Pallasch, Christian P. ORCID: 0000-0001-5675-6905 (2025). Protocol for assessing antibody-mediated cellular phagocytosis of lymphoma cells by human and murine macrophages in vitro. STAR Protocols, 6 (4). pp. 1-16. Elsevier. ISSN 2666-1667

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Abstract

[Artikel-Nr.: 104126] Antibody-dependent phagocytosis is an important process of cell engulfment and degradation by macrophages that holds therapeutical importance in the context of chemo-immunotherapy in, for example, lymphoma. Here, we present an in vitro protocol for determining the antibody-dependent phagocytosis of lymphoma cells by different types of macrophages under different treatment conditions. We describe steps for generating mature macrophages from different sources, usage of different clinically established antibodies, and elucidating the driving cell type of the observed changes in phagocytosis.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Beielstein, Anna C. UNSPECIFIED https://orcid.org/0000-0001-7710-1377 UNSPECIFIED Blakemore, Stuart UNSPECIFIED https://orcid.org/0000-0003-2892-1162 UNSPECIFIED Pallasch, Christian P. UNSPECIFIED https://orcid.org/0000-0001-5675-6905 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-812626
Identification Number:	10.1016/j.xpro.2025.104126
Journal or Publication Title:	STAR Protocols
Volume:	6
Number:	4
Page Range:	pp. 1-16
Number of Pages:	16
Date:	19 December 2025
Publisher:	Elsevier
ISSN:	2666-1667
Language:	English
Faculty:	Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases Faculty of Medicine > Innere Medizin > Klinik I für Innere Medizin - Hämatologie und Onkologie Zentrum für Molekulare Medizin
Subjects:	Medical sciences Medicine
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81262