Universität zu Köln

Moormann, Jannis ORCID: 0000-0002-4059-2480, Heinemann, Björn ORCID: 0000-0002-3962-4174, Angermann, Cecile ORCID: 0000-0002-1051-5679, Koprivova, Anna ORCID: 0000-0001-8168-4536, Armbruster, Ute ORCID: 0000-0002-8814-8207, Kopriva, Stanislav ORCID: 0000-0002-7416-6551 and Hildebrandt, Tatjana M. (2025). Cysteine Signalling in Plant Pathogen Response. Plant, Cell & Environment, 48 (10). pp. 7107-7122. Wiley. ISSN 0140-7791

PDF Plant Cell Environment - 2025 - Moormann - Cysteine Signalling in Plant Pathogen Response.pdf Bereitstellung unter der CC-Lizenz: Creative Commons Attribution. Download (2MB)

Abstract

The amino acid cysteine is the precursor for a wide range of sulfur-containing functional molecules in plants, including enzyme cofactors and defence compounds. Due to its redox active thiol group cysteine is highly reactive. Synthesis and degradation pathways are present in several subcellular compartments to adjust the intracellular cysteine concentration. However, stress conditions can lead to a transient increase in local cysteine levels. Here we investigate links between cysteine homeostasis and metabolic signalling in Arabidopsis thaliana. The systemic proteome response to cysteine feeding strongly suggests that Arabidopsis seedlings interpret accumulation of cysteine above a certain threshold as a signal for a biotic threat. Cysteine supplementation of Arabidopsis plants via the roots increases their resistance to the hemibiotrophic bacterium Pseudomonas syringae confirming the protective function of the cysteine induced defence pathways. Analysis of mutant plants reveals that the balance of cysteine synthesis between the cytosol and organelles is crucial during Arabidopsis immune response to Pseudomonas syringae. The induction profile of pathogen responsive proteins by cysteine provides insight into potential modes of action. Our results highlight the role of cysteine as a metabolic signal in the plant immune response and add evidence to the emerging concept of intracellular organelles as important players in plant stress signalling.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Moormann, Jannis UNSPECIFIED https://orcid.org/0000-0002-4059-2480 UNSPECIFIED Heinemann, Björn UNSPECIFIED https://orcid.org/0000-0002-3962-4174 UNSPECIFIED Angermann, Cecile UNSPECIFIED https://orcid.org/0000-0002-1051-5679 UNSPECIFIED Koprivova, Anna UNSPECIFIED https://orcid.org/0000-0001-8168-4536 UNSPECIFIED Armbruster, Ute UNSPECIFIED https://orcid.org/0000-0002-8814-8207 UNSPECIFIED Kopriva, Stanislav UNSPECIFIED https://orcid.org/0000-0002-7416-6551 UNSPECIFIED Hildebrandt, Tatjana M. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-812977
Identification Number:	10.1111/pce.70017
Journal or Publication Title:	Plant, Cell & Environment
Volume:	48
Number:	10
Page Range:	pp. 7107-7122
Number of Pages:	16
Date:	16 October 2025
Publisher:	Wiley
ISSN:	0140-7791
Language:	English
Faculty:	Faculty of Mathematics and Natural Sciences
Divisions:	CEPLAS - Cluster of Excellence on Plant Sciences Faculty of Mathematics and Natural Sciences > Department of Biology > Botanical Institute
Subjects:	Life sciences
Uncontrolled Keywords:	Keywords Language amino acid metabolism ; immunometabolism ; infochemicals ; mitochondria ; proteomics ; sulfur signalling UNSPECIFIED
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81297