Universität zu Köln

Kim, Soya, Shahab, Jaffer, Vogelsang, Elisabeth and Wodarz, Andreas ORCID: 0000-0002-0007-6289 (2024). Re-assessment of the subcellular localization of Bazooka/Par-3 in Drosophila : no evidence for localization to the nucleus and the neuromuscular junction. Biology Open, 13 (6). pp. 1-9. Company of Biologists Limited. ISSN 2046-6390

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Abstract

[Artikel-Nr. bio060544] Bazooka/Par-3 (Baz) is an evolutionarily conserved scaffold protein that functions as a master regulator for the establishment and maintenance of cell polarity in many different cell types. In the vast majority of published research papers Baz has been reported to localize at the cell cortex and at intercellular junctions. However, there have also been several reports showing localization and function of Baz at additional subcellular sites, in particular the nuclear envelope and the neuromuscular junction. In this study we have re-assessed the localization of Baz to these subcellular sites in a systematic manner. We used antibodies raised in different host animals against different epitopes of Baz for confocal imaging of Drosophila tissues. We tested the specificity of these antisera by mosaic analysis with null mutant baz alleles and tissue-specific RNAi against baz. In addition, we used a GFP-tagged gene trap line for Baz and a bacterial artificial chromosome (BAC) expressing GFP-tagged Baz under control of its endogenous promoter in a baz mutant background to compare the subcellular localization of the GFP-Baz fusion proteins to the staining with anti-Baz antisera. Together, these experiments did not provide evidence for specific localization of Baz to the nucleus or the neuromuscular junction.

Item Type:	Article
Creators:	Creators Email ORCID ORCID Put Code Kim, Soya UNSPECIFIED UNSPECIFIED UNSPECIFIED Shahab, Jaffer UNSPECIFIED UNSPECIFIED UNSPECIFIED Vogelsang, Elisabeth UNSPECIFIED UNSPECIFIED UNSPECIFIED Wodarz, Andreas UNSPECIFIED https://orcid.org/0000-0002-0007-6289 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-813721
Identification Number:	10.1242/bio.060544
Journal or Publication Title:	Biology Open
Volume:	13
Number:	6
Page Range:	pp. 1-9
Number of Pages:	9
Date:	15 June 2024
Publisher:	Company of Biologists Limited
ISSN:	2046-6390
Language:	English
Faculty:	Faculty of Medicine
Divisions:	CECAD - Cluster of Excellence Cellular Stress Responses in Aging-Associated Diseases Faculty of Medicine > Anatomie > Zentrum für Anatomie Zentrum für Molekulare Medizin
Subjects:	Medical sciences Medicine
Uncontrolled Keywords:	Keywords Language Bazooka/Par-3 ; Nuclear localization ; Neuromuscular junction ; Subcellular localization English
['eprint_fieldname_oa_funders' not defined]:	Publikationsfonds UzK
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/81372